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Key Documents

ABF29

Sigma-Aldrich

Anti-NLRP6 (NALP6) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

NACHT, PYRIN-containing APAF1-like protein 5, LRR and PYD domains-containing protein 6

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

species reactivity (predicted by homology)

rhesus macaque (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_612202

UniProt accession no.

P59044

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NLRP6(171389)

General description

NRLP6 (NALP6) (also known as PYRIN-containing Apaf-1-like protein 5 or PYPAF5) belongs to a family of proteins that play a critical role in apoptosis and in inflammatory signaling. Its expression is highly restricted to granulocytes and T-cells. Co-expression of NALP6 with ASC results in synergistic activation of caspase-1-dependent cytokine processing, subsequent NF-kB activation, and downstream recruitment of PYPAF5 to punctate structures in the cytoplasm. Overlapping genes in NALP6/PYPAF5 locus encode two V2-type vasopressin isoreceptors: angiotensin-vasopressin receptor (AVR) and non-AVR.

Specificity

Other homologies: Mouse (93% sequence homology).
This antibody recognizes the NACHT domain of NLRP6 (NALP6).

Immunogen

Epitope: NACHT domain
Linear peptide corresponding to NACHT domain of human NLRP6 (NALP6).

Application

Anti-NLRP6 (NALP6) Antibody detects level of NLRP6 (NALP6) & has been published & validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NLRP6 (NALP6) in epithelial tissue of proximal and distal tubules of human kidney, and in human bone marrow tissue.

Quality

Evaluated by Western Blot in rat basophilic leukemia (RBL) cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected NLRP6 (NALP6) in 10 µg of rat basophilic leukemia (RBL) cell lysate.

Target description

~99 kDa observed

Analysis Note

Control
Rat basophilic leukemia (RBL) cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pierre-Gregoire Coulon et al.
Frontiers in immunology, 10, 1631-1631 (2019-08-02)
The crosstalk between the host's inflammasome system and the invading virulent/less-virulent viruses determines the outcome of the ensuing inflammatory response. An appropriate activation of inflammasomes triggers antiviral inflammatory responses that clear the virus and heal the inflamed tissue. However, an
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Abdallah Mound et al.
PloS one, 18(1), e0279028-e0279028 (2023-01-21)
Nod-Like Receptor Pyrin domain-containing protein 6 (NLRP6), a member of the Nucleotide-oligomerization domain-Like Receptor (NLR) family of proteins, assembles together with the ASC protein to form an inflammasome upon stimulation by bacterial lipoteichoic acid and double-stranded DNA. Besides its expression
David Furman et al.
Nature medicine, 23(2), 174-184 (2017-01-17)
Low-grade, chronic inflammation has been associated with many diseases of aging, but the mechanisms responsible for producing this inflammation remain unclear. Inflammasomes can drive chronic inflammation in the context of an infectious disease or cellular stress, and they trigger the

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