AB4330

Anti-DDX4 (Vasa) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): DEAD (Asp-Glu-Ala-Asp) box polypeptide 4, probable ATP-dependent RNA helicase DDX4, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4, Vasa homolog, DEAD box protein 4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human, mouse
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NP_034159
• UniProt accession no.: Q61496
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DDX4(54514)

Description

General description

DDX4 (Vasa or MVH) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. DDX4 (Vasa) proteins are mostly localized in testicular germ cells in the cytoplasm of spermatocytes. DDX4 (Vasa) is a high-copy extragenic suppressor gene. DDX4 (Vasa) is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility.

Specificity

This antibody recognizes DDX4 (Vasa) at the C-terminus.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of mouse DDX4 (Vasa).

Application

Anti-DDX4 (Vasa) Antibody detects level of DDX4 (Vasa) & has been published & validated for use in WB, IH(P).

Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected DDX4 (Vasa) in normal human testis tissue.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

RNA Metabolism & Binding Proteins

Quality

Evaluated by Western Blot in mouse testis tissue lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected DDX4 (Vasa) in 10 µg of mouse testis tissue lysate. Western Blot performed without Tween.

Target description

~76 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse testis tissue lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable