AB1591P
Anti-Dopamine Transporter Antibody
Chemicon®, from rabbit
Synonym(s):
DAT
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SLC6A3(6531)
General description
Plasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinson′s disease, Tourette′s disease, schizophrenia, and addiction. It is a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm.
Specificity
Recognizes Dopamine transporter (DAT). By Western blot the antibody recognizes a broad 80 kDa band in rat striatum (Lew et al. 1992; Freed et al. 1995; Vaughan et al. 1996) and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55 kDa.
Immunogen
An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (Kilty et al. 1991; Giros et al. 1991; Shimada et al. 1991; Kitayma 1992). This region is presumably the cytoplasmic domain.
Application
Detect Dopamine Transporter using this Anti-Dopamine Transporter Antibody validated for use in ELISA, IH, IP & WB.
Western blot: 1-10 μg/mL using ECL. An antibody raised against a similar peptide has been shown to react with a broad 75-80 kDa band in rat striatum (Hastrup et al., 2001) and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55 kDa.
Immunoprecipitation: 2-10 μg of antibody (Chang et al., 2001)
Immunohistochemistry: 1:250-1:500 on frozen sections of tissues fixed with 4% paraformaldehyde (Chang et al., 2001; Ourednik, et al., 2002). 0.05% trypsin for 10-15min has been found effective in exposing the epitope in some preparations.
ELISA: 0.5-1.0 μg/mL using 1 μg/mL of DAT control peptide (Catalog Number AG296) per well.
Optimal working dilutions must be determined by end user.
Immunoprecipitation: 2-10 μg of antibody (Chang et al., 2001)
Immunohistochemistry: 1:250-1:500 on frozen sections of tissues fixed with 4% paraformaldehyde (Chang et al., 2001; Ourednik, et al., 2002). 0.05% trypsin for 10-15min has been found effective in exposing the epitope in some preparations.
ELISA: 0.5-1.0 μg/mL using 1 μg/mL of DAT control peptide (Catalog Number AG296) per well.
Optimal working dilutions must be determined by end user.
Target description
80 kDa
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jian Huang et al.
Brain and behavior, 10(2), e01533-e01533 (2020-01-17)
This study aims to establish a methamphetamine (METH)-induced behavioral sensitization model using tree shrews, as well as to measure the protein expression of the dopamine D3 receptor (D3R) and dopamine transporter (DAT). Forty tree shrews were equally and randomly divided
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Behavioural brain research, 339, 153-168 (2017-11-29)
Alpha-Synuclein (α-Syn) accumulation is considered a major risk factor for the development of synucleinopathies such as Parkinson's disease (PD) and dementia with Lewy bodies. We have generated mice overexpressing full-length human α-Syn fused to a membrane-targeting signal sequence under the
Kirill Shumilov et al.
PloS one, 13(8), e0203135-e0203135 (2018-08-30)
The striatum is a complex structure in which the organization in two compartments (striosomes and matrix) have been defined by their neurochemical profile and their input-output connections. The striosomes receive afferences from the limbic brain areas and send projections to
Chisayo Kozuka et al.
British journal of pharmacology, 172(18), 4519-4534 (2015-07-04)
γ-Oryzanol, derived from unrefined rice, attenuated the preference for dietary fat in mice, by decreasing hypothalamic endoplasmic reticulum stress. However, no peripheral mechanisms, whereby γ-oryzanol could ameliorate glucose dyshomeostasis were explored. Dopamine D2 receptor signalling locally attenuates insulin secretion in
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