203390

(±)-Blebbistatin

≥97% (HPLC), solid, Myosin II inhibitor, Calbiochem^®

• Synonym(s): (±)-Blebbistatin
• Empirical Formula (Hill Notation): C₁₈H₁₆N₂O₂
• CAS Number: 674289-55-5
• Molecular Weight: 292.33
• MDL number: MFCD04113775
• UNSPSC Code: 12352200
• NACRES: NA.77
• Assay: ≥97% (HPLC)
• Form: solid
• Storage condition: OK to freeze, protect from light

Properties

• Product Name: (±)-Blebbistatin, (±)-Blebbistatin, CAS 674289-55-5, is a cell-permeable, selective, and reversible inhibitor of nonmuscle myosin II. Blocks cell blebbing and disrupts cell migration & cytoKinesis in vertebrate cells.
• Quality Segment: 100
• assay: ≥97% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: yellow
• solubility: methanol: 1.5 mg/mL, 100% DMSO: 100 mg/mL, 90% DMSO: 75 mg/mL
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: N2(CCC3(C2=Nc4c(cc(cc4)C)C3=O)O)c1ccccc1
• InChI: 1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3
• InChI key: LZAXPYOBKSJSEX-UHFFFAOYSA-N

Description

General description

A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of nonmuscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤ 100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly. A 50 mM (5 mg/342 µl) solution of (±)-Blebbistatin (Cat. No. 203389 ) in 90% DMSO is also available.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
ATPase

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: <100 µM inhibiting the ATPase and gliding motility of human platelets

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution in 90% DMSO, aliquot and freeze (-20°C). Stock solutions in 90% DMSO are stable for up to 1 month at -20°C. Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.

Other Notes

Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl.12, 271a.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable