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  • SMAD3 Regulates Follicle-stimulating Hormone Synthesis by Pituitary Gonadotrope Cells

SMAD3 Regulates Follicle-stimulating Hormone Synthesis by Pituitary Gonadotrope Cells

The Journal of biological chemistry (2016-12-21)
Yining Li, Gauthier Schang, Ulrich Boehm, Chu-Xia Deng, Jonathan Graff, Daniel J Bernard
PMID27994055
ABSTRACT

Pituitary follicle-stimulating hormone (FSH) is an essential regulator of fertility in females and of quantitatively normal spermatogenesis in males. Pituitary-derived activins are thought to act as major stimulators of FSH synthesis by gonadotrope cells.

MATERIALS
Product Number
Brand
Product Description
P6148
Sigma-Aldrich
Paraformaldehyde, reagent grade, crystalline
T48402
Sigma-Aldrich
2,2,2-Tribromoethanol, 97%
A4378
Sigma-Aldrich
Bovine Serum Albumin, lyophilized powder, 1× crystallized, ≥97% (agarose gel electrophoresis)
C1063
Sigma-Aldrich
Chorionic gonadotropin human, lyophilized powder, vial of ~2,500 IU
H3884
Sigma-Aldrich
Hyaluronidase from bovine testes, Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid
M7653
Sigma-Aldrich
Medium 199, With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture