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G005959

HPLC Analysis of Glycan and Isomers on Proteomix® SAX-NP5, High Resolution Separation

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 μm particles (Z777228)
mobile phase: (A) 2.5% (v/v) acetic acid, 0.5% TEA in water; 0.5% acetic acid in acetonitrile
gradient: 0-100% B in 60 min
flow rate: 0.3 mL/min
detector: Fluorescence, Ex/Em=360/425 nm
sample: 2-AA (anthranilic acid) labeled N-linked oligosaccharide profiling of an IgG1 sample

suitability

application for HPLC

application(s)

life science and biopharma

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Proteomix is a registered trademark of Sepax Technologies

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