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G005604

HPLC Analysis of Herbal Supplement Containing Milk Thistle on Ascentis® Express 2.7 μm C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] water with 0.1% formic acid; [B] methanol
gradient: held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min
flow rate: 0.6 mL/min
pressure: 4105 psi (283 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 20 μL
sample: 20 mg/mL in water:ethanol (5:95); sonicate 15 minutes; filter 0.45 μm; dilute to water:ethanol (80:20)

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle containing herbal supplement.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Taxifolin
    Silychristin
    Apigenin 7-glucoside
    Silydianin
    Quercitrin
    Silibinin
    Silybin B
    Isosilybin A
    Isosilybin B
    See All (9)

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