The maximum amount depends on the composition of the matrix besides the lipid and the specific objective. As a general guideline, it's advisable not to load more than 5% of the sorbent mass. Therefore, with a 500 mg bed mass, it would be best to start with 25 mg of lipid. The procedure can then be adjusted as needed to achieve the desired outcome.
54059-U
Supelclean™ LC-NH2 SPE Tube
bed wt. 500 mg, volume 6 mL, pk of 30
Synonym(s):
NH2 (amine) SPE cartidge, 6 mL
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30 EA
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30 EA
£124.10
About This Item
UNSPSC Code:
41115712
NACRES:
NB.21
£124.10
List Price£146.00Save 15%Please contact Customer Service for Availability
Request a Bulk OrderRequest a Sample
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material
polypropylene hardware
Quality Level
product line
Supelclean™
composition
bed wt., 500 mg
packaging
pk of 30
extent of labeling
5% C loading
technique(s)
solid phase extraction (SPE): suitable
surface area
475 m2/g
volume
6 mL
matrix
silica gel base material (irregularly shaped)
matrix active group
amino bonding
particle size
45 μm
pore size
0.8 cm3/g pore volume
60 Å pore size
pKa
9.8
application(s)
food and beverages
separation technique
normal phase
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General description
Supelclean™ LC-NH2 SPE tube is ideally used for the normal phase extraction of polar to moderately polar compounds from non-aqueous samples.
Legal Information
Supelclean is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
>464.0 °F
Flash Point(C)
> 240 °C
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What is the maximum amount of total lipid that can be loaded onto column 54059-U without overloading, for example, can 250mg total lipid be loaded?
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