Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express F5 HPLC columns is 3.5 micromoles/meter-squared.
53577-U
Ascentis® Express 90 Å F5 (2.7 μm) HPLC Columns
L × I.D. 7.5 cm × 3 mm, HPLC Column
Synonym(s):
Core-shell (SPP) Fused Core PFP HPLC column
Sign Into View Organizational & Contract Pricing
Select a Size
1 EA
£648.00
£648.00
Please contact Customer Service for Availability
All Photos(1)
Select a Size
Change View
1 EA
£648.00
About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × I.D.:
7.5 cm × 3 mm
surface area:
135 m2/g
particle size:
2.7 μm
matrix active group:
PFP (pentafluorophenyl) phase
pore size:
90 Å pore size
matrix:
Fused-Core particle platform
superficially porous particle
superficially porous particle
£648.00
Please contact Customer Service for Availability
Product Name
Ascentis® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 7.5 cm × 3 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L43
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
60 °C temp. range
600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
7.5 cm × 3 mm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform
superficially porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
2.7 μm
pore size
90 Å pore size
operating pH
2-8
application(s)
food and beverages
separation technique
reversed phase
Looking for similar products? Visit Product Comparison Guide
General description
The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Application
- Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study utilizes the Ascentis® Express F5, 2.7 µm HPLC Column to develop a robust method for the determination of lumacaftor in the presence of ivacaftor, identifying five novel degradation products. The high resolution and efficiency of the column were crucial for the detailed analysis and identification process. (Özcan et al., 2023).
- Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: The Ascentis® Express F5 column was employed to develop stability-indicating methods for tasimelteon, ensuring accurate determination and high-resolution identification of degradation products, highlighting the column′s effectiveness in stability studies and complex sample matrices. (Özcan et al., 2020).
- Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review discusses advancements in ultra-fast HPLC, focusing on new generation superficially porous silica columns like the Ascentis® Express F5. The article elaborates on the performance improvements and applications in analytical chemistry, emphasizing the column′s role in enhancing speed and resolution. (Ali et al., 2012).
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
guard cartridge
Product No.
Description
Pricing
related product
required but not provided
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore
Articles
Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds
Protocols
Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®
-
What is the carbon load for Ascentis® Express F5 HPLC Columns?
1 answer-
Helpful?
-
-
Are guard columns available for Ascentis® Express F5 3.0 mm ID HPLC Columns?
1 answer-
Yes, we have guard columns available for product Ascentis® Express F5 3.0 mm ID HPLC Columns. Product 53500-U is the Ascentis® Express Guard Holder and Product 53597-U Ascentis® Express F5 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5 mm × I.D. 3.0 mm .
Helpful?
-
-
Can Ascentis® Express F5 HPLC columns be used for LC-MS?
1 answer-
Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis® Express F5 HPLC Columns for high throughput UHPLC and LC-MS are that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
Helpful?
-
-
What flow rate should I use with Ascentis® Express F5 HPLC columns?
1 answer-
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis® Express F5 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
Helpful?
-
-
Can I use Ascentis® Express F5 HPLC Columns on any type of HPLC system?
1 answer-
Ascentis® Express F5 HPLC Columns HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. These columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
Helpful?
-
-
What is the pressure rating for Ascentis® Express F5 HPLC Columns?
1 answer-
Ascentis® Express F5 HPLC Columns are stable to operating pressures up to 600 bar (9000 psi).
Helpful?
-
-
Do I need special fittings and tubing to connect Ascentis® Express F5 HPLC Columns?
1 answer-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Is there anything special I need to do to my HPLC system to use Ascentis® Express F5 HPLC Columns?
1 answer-
Nothing special is required to use Ascentis® Express F5 HPLC Columns. To obtain the full benefits of Ascentis® Express Columns, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
Helpful?
-
-
How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 answer-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
