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publication info
S. Marsh, Humana Press, 2007, 208 pp., hard cover
General description
This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5–7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8–13, and the important aspects of data storage is discussed in Chapter 14. This text both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.
Table of Contents
1. The History of Pyrosequencing
2. Pyrosequencing Applications
3. Web Based Primer Design Software for Genome Scale Genotyping by Pyrosequencing
4. Pyrosequencing: A Simple Methods for Accurate Genotyping
5. Universal Primer Applications for Pyrosequencing
6. Allele Quantification and DNA Pooling Methods
7. Multiplex Pyrosequencing for DNA Variation Analysis
8. Analysis of Gene Specific DNA Methylation Patterns by Pyrosequencing Technology
9. Detecting Mutations That Confer Oxazolidinone Resistance in Gram Positive Bacteria
10. Pyrosequencing Based Strategies for Improved Allele Typing of Human Leukocute Antigen Loci
11. Pyrosequencing of Phage Display Libraries for the Identification of Cell Specific Targeting Libraries
12. Gene Copy Number Detection in Animal Studies
13. Detection of Allelic Imbalance in Gene Expression
14. Pyrosequecning Genotype Storage Techniques
Index
2. Pyrosequencing Applications
3. Web Based Primer Design Software for Genome Scale Genotyping by Pyrosequencing
4. Pyrosequencing: A Simple Methods for Accurate Genotyping
5. Universal Primer Applications for Pyrosequencing
6. Allele Quantification and DNA Pooling Methods
7. Multiplex Pyrosequencing for DNA Variation Analysis
8. Analysis of Gene Specific DNA Methylation Patterns by Pyrosequencing Technology
9. Detecting Mutations That Confer Oxazolidinone Resistance in Gram Positive Bacteria
10. Pyrosequencing Based Strategies for Improved Allele Typing of Human Leukocute Antigen Loci
11. Pyrosequencing of Phage Display Libraries for the Identification of Cell Specific Targeting Libraries
12. Gene Copy Number Detection in Animal Studies
13. Detection of Allelic Imbalance in Gene Expression
14. Pyrosequecning Genotype Storage Techniques
Index
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