Skip to Content
Merck
All Photos(1)

Documents

U8501

Sigma-Aldrich

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast

Type X, lyophilized powder, ≥40 units/mg protein

Synonym(s):

UTP:α-D-glucose-1-phosphate uridylyltransferase

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9026-22-6
Enzyme Commission number:
2.7.7.9 (BRENDA, IUBMB)
EC Number:
232-811-2
MDL number:
MFCD00132561
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bakers yeast

Quality Level

200

type

Type X

form

lyophilized powder

specific activity

≥40 units/mg protein

composition

Protein, 30-60% modified Warburg-Christian

foreign activity

UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%
inorganic pyrophosphatase ≤0.5%

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.

Application

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:
  • in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-13C9
  • to quantify Suc synthase (SUS) enzyme activity in rice
  • to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes

Biochem/physiol Actions

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg2+, Ca2+, Mn2+, Ni2+ for its activity.

Unit Definition

One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.

Physical form

Lyophilized, sulfate-free powder containing citrate buffer salt

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Activities of enzymes for sucrose-starch conversion in developing endosperm of rice and their association with grain filling in extra-heavy panicle types
Kato T, et al.
Plant Protection Science, 10(4), 442-450 (2007)
Ziying Huang et al.
Molecular biology reports, 38(7), 4291-4302 (2010-11-30)
OsUgp2, a rice UDP-glucose pyrophosphorylase gene, has previously been shown to preferentially express in maturing pollens and plays an important role in pollen starch accumulation. Here, a 1943 bp promoter fragment (P1943) of OsUgp2 was characterized by 5' deletion and
Orelvis Portal et al.
Plant cell reports, 30(5), 913-928 (2011-02-01)
Mycosphaerella fijiensis, a hemibiotrophic fungus, is the causal agent of black leaf streak disease, the most serious foliar disease of bananas and plantains. To analyze the compatible interaction of M. fijiensis with Musa spp., a suppression subtractive hybridization (SSH) cDNA
Expression of trehalose-6-phosphate phosphatase in maize ears improves yield in well-watered and drought conditions
Michael L N, et al.
Nature Biotechnology, 862?869-862?869 (2015)
Kieran Smallbone et al.
Methods in enzymology, 500, 355-370 (2011-09-29)
In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway based on kinetic data from experimental measurements and literature review. Our methodology is presented by utilizing the example of trehalose metabolism in yeast.
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service