T1323

Monoclonal Anti-ε-Tubulin antibody produced in mouse

clone TUB-11, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD03792420
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: TUB-11, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 55 kDa
• species reactivity: frog, mouse, chicken, human
• technique(s): immunohistochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 2-4 μg/mL using whole extract of cultured chicken fibroblasts
• isotype: IgG2a
• UniProt accession no.: Q9UJT0
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBE1(51175) ; mouse ... Tube1(71924)

Description

General description

Monoclonal Anti-ε-Tubulin (mouse IgG2a isotype) is derived from the TUB-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide.

Tubulin epsilon 1 (ε-tubulin) is a member of tubulin superfamily. ε-tubulin is localized to the centrosome in cell-cycle-dependent manner. It is encoded by the gene mapped to human chromosome 6q21.

Specificity

Monoclonal Anti-ε-Tubulin recognizes human, mouse, frog, and chicken ε-tubulin (55 kDa).

Immunogen

synthetic peptide corresponding to amino acids 352-366 of human ε-tubulin, conjugated to KLH.

Application

Monoclonal Anti-ε-Tubulin antibody produced in mouse may be used in:

• immunoblotting
• enzyme-linked immunosorbent assay (ELISA)
• immunofluorescence

Biochem/physiol Actions

Tubulin ε 1 (ε-Tubulin) plays a vital role in microtubule anchoring and centriole maturation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at−20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.