SRP0718
TRKA (G595R), Active
PRECISIO® Kinase, recombinant
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
recombinant
expressed in baculovirus infected Sf9 cells
Assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
2.0-3.0 nmol/min·mg
mol wt
~66 kDa
concentration
0.05 μg/μL
accession no.
NM_002529
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... TRKA(4914)
Application
Useful for kinase assay.
Biochem/physiol Actions
Recombinant human TRKA (G595R) (440-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKA is a member of the trk proto-oncogene family and encodes a 140kDa, membrane-spanning protein tyrosine kinase that is the functional receptor for nerve growth factor (NGF). NGF elicits the rapid phosphorylation of gp140trk on tyrosine residues leading to increased c-Fos expression, DNA synthesis and morphologic transformation (1). A decreased expression of TRKA on the striatal cholinergic neurons has been observed which may contribute, when it reaches a crucial threshold, to the death of cholinergic neurons observed in Alzheimer disease (2).
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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