SAB4700290

Monoclonal Anti-CD42b antibody produced in mouse

clone HIP1, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-GP1BA, Anti-GPIb α
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: HIP1, monoclonal
• form: buffered aqueous solution
• species reactivity: human, nonhuman primates
• concentration: 1 mg/mL
• technique(s): flow cytometry: suitable
• isotype: IgG1
• NCBI accession no.: NM_000173.5
• UniProt accession no.: P07359
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... GP1BA(2811)

Description

General description

Cluster of differentiation 42b (CD42b), also known as, glycoprotein Ib platelet α subunit (GP1BA), is encoded by the gene mapped to human chromosome 17p13.2.

The antibody HIP1 reacts with CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.

Immunogen

Peripheral blood mononuclear cells of a patient suffering with CLL

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 2-8 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Biochem/physiol Actions

Cluster of differentiation 42b (CD42b) clustering promotes integrin αIIbβ3-dependent platelet aggregation, phagocytosis and rapid platelet clearance in the liver. The encoded protein acts as a platelet receptor for von Willebrand factor (VWF) and other ligands present in circulation. Mutation in the gene leads to autosomal recessive Bernard-Soulier syndrome. CD42b interacts with plasma hemoglobin (Hb) and enhances platelet activation and apoptosis in a concentration-dependent manner and also regulates platelet functions.

Features and Benefits

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Physical form

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable