SAB4300512

Anti-NF2 (Ab-518) antibody produced in rabbit

affinity isolated antibody

• Synonym(s): Anti-ACN antibody produced in rabbit, Anti-BANF antibody produced in rabbit, Anti-SCH antibody produced in rabbit, Anti-neurofibromin 2 (merlin) antibody produced in rabbit
• MDL number: MFCD03845618
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~69 kDa
• species reactivity: mouse, human, rat
• concentration: 1 mg/mL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100; western blot: 1:500-1:1000
• isotype: IgG
• immunogen sequence: (R-L-S-M-E)
• NCBI accession no.: NP_000259.1
• UniProt accession no.: P35240
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NF2(4771)

Description

General description

Neurofibromin 2 (NF2) encodes the protein Merlin, which belongs to Ezrin/Radixin/Moesin (ERM) family of proteins. NF2 is a cytoskeletal protein involved in stabilization of microtubules. NF2 has four-point-one, ezrin, radixin, moesin (FERM) domain in the N-terminal, a coiled-coil domain, and does not contain C-terminal actin-binding motif like other ERM proteins. In human chromosome, the gene NF2 is localized on 22q12.2

Immunogen

Peptide sequence around aa. 516-520 (R-L-S-M-E), according to the protein NF2.

Biochem/physiol Actions

Neurofibromin 2 (NF2) is a tumour suppressor. NF2 translocates to nucleus and binds to CRL4DCAF1 (Cullin Ring E3 ubiquitin ligase cullin 4 (CRL4) DDB1 (DNA damage binding protein 1) and CUL4 (cullin 4) associated factor 1 (DCAF1)) and suppresses tumour growth. The gene NF2 is inactivated in the familial neurofibromatosis type 2 cancer syndrome. Mutations of NF2 is associated with multiple tumours like Schwannomas, meningiomas, and malignant mesotheliomas. NF2 regulates Hippo signalling pathway and further activates yes associated protein 1 (YAP-1) and modulates cell growth in neurofibromatosis 2. Molecular modifications in NF2 gene leads to liver cancer through the involvement of Hippo pathway of pathogenesis. Polymorphism in NF2 is related to medulloblastoma.

Features and Benefits

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Target description

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable