SAB4200415

Anti-NEZHA antibody, Mouse monoclonal

clone NEZHA-1, purified from hybridoma cell culture

• Synonym(s): Anti-CAMSAP3, Anti-Calmodulin regulated spectrin-associated protein family member 3, Anti-KIAA, Monoclonal Anti-NEZHA antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: NEZHA-1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~150 kDa
• species reactivity: canine, human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): suitable; indirect immunofluorescence: suitable; western blot: 2.5-5.0 μg/mL using whole extracts of human SW480 cells
• isotype: IgG1
• UniProt accession no.: Q9P1Y5
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CAMSAP3(57662) ; mouse ... Camsap3(69697)

Description

General description

Monoclonal Anti-NEZHA (mouse IgG1 isotype) is derived from the hybridoma NEZHA-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. Nezha (a deity in Chinese mythology) also known as KIAA1543 and calmodulin-regulated spectrin associated protein, is a 1276 amino acid protein that contains one calponin homology (CH) and two coiled coil (CC1 and 2) domains, as well as one DUF1781 (DUF) domain of unknown function. NEZHA is mapped to human chromosome 19p13.

NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) contains a conserved domain called cholecystokinin (CKK) domain at carboxy-terminal, as well as several coiled-coil regions and an calponin homology domain at amino-terminal.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and dog NEZHA.

Application

Monoclonal Anti-NEZHA antibody produced in mouse has been used in immunofluorescence staining.

Monoclonal Anti-NEZHA antibody produced in mouse has been used in:

• immunofluorescence
• immunoprecipitation
• immunofluorescence
• mass-spectrometry-based analysis of a streptavidin pull-down assay
• immunostaining

Biochem/physiol Actions

CAMSAP3 (calmodulin-regulated spectrin-associated protein 3)/ NEZHA modulates the minus-end dynamics of microtubules. It helps to maintain neuronal polarity. Absence of this gene results in the formation of supernumerary axon.

NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) facilitates translocation of Golgi vesicles in epithelial cells. In addition, it also helps in positioning the apical-to-basal polarity of microtubules in epithelial cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable