SAB4200384

Anti-SMAD6 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-MADH6, Anti-MADH7, Anti-SMAD family member 6
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: human, mouse
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells over-expressing human SMAD6; indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells; western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human SMAD6
• UniProt accession no.: O43541
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SMAD6(4091) ; rat ... Smad6(367100)

Description

General description

SMAD6 is a member of inhibitory Smads (I-Smad) family. It consists of two conserved domains, the N-terminal MH1 and C-terminal MH2, that are separated by a proline-rich linker region of varying length.

Immunogen

peptide corresponding to the N-terminal region of human SMAD6, conjugated to KLH. The corresponding sequence is identical in rat and differs by a single amino acid in mouse.

Application

Anti-SMAD6 (N-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

SMAD6 plays an important role in transforming growth factor-β (TGF-β) pathway. It regulates cell proliferation, apoptosis and differentiation. SMAD6 is a key negative regulator of bone morphogenetic protein (BMP) signaling in the cytoplasm and in the nucleus. SMAD6 inhibits SMAD signaling in the nucleus by interacting with transcriptional repressors such as HOXC-8, class I histone deacetylases (HDACs) and transcriptional corepressor C-terminal binding protein (CtBp).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable