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S9626

Sigma-Aldrich

Sulfatase from Helix pomatia

Type H-1, sulfatase ≥10,000 units/g solid

Synonym(s):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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5000 UNITS
£48.50
10000 UNITS
£65.00
100000 UNITS
£323.00

About This Item

CAS Number:
9016-17-5
Enzyme Commission number:
3.1.6.1 (BRENDA, IUBMB)
EC Number:
232-772-1
MDL number:
MFCD00132399
UNSPSC Code:
12352204
NACRES:
NA.54

£48.50

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type

Type H-1

Quality Level

200

form

powder

sulfatase activity

≥10,000 units/g solid

secondary activity

≥300 unit/mg solid β-glucuronidase (at pH 5.0)

mol wt

~85 kDa by gel filtration

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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Application

Sulfatase from Helix pomatia has been used in:
  • sample extraction[1]
  • sulfatase assays[2]
  • enzymatic hydrolysis[3]
  • extract preparation for glucosinolate analysis[4]

Biochem/physiol Actions

Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates.[5] It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.[6]

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000389811
0000381301
0000363885
0000377335
0000376660

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Ivica Blažević et al.
Phytochemistry, 169, 112100-112100 (2019-11-28)
The glucosinolates (GSLs) is a well-defined group of plant metabolites characterized by having an S-β-d-glucopyrano unit anomerically connected to an O-sulfated (Z)-thiohydroximate function. After enzymatic hydrolysis, the sulfated aglucone can undergo rearrangement to an isothiocyanate, or form a nitrile or
A single monooxygenase, ese, is involved in the metabolism of the organochlorides endosulfan and endosulfate in an Arthrobacter sp.
Weir K M, et al.
Applied and Environmental Microbiology, 72(5), 3524-3530 (2006)
Stephen S Hecht et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 13(6), 997-1004 (2004-06-09)
Vegetable consumption, including cruciferous vegetables, is protective against lung cancer, but the mechanisms are poorly understood. The purpose of this study was to investigate the effects of cruciferous vegetable consumption on the metabolism of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)
Use of diabetic test kits to assess the recovery of glucosinolates during isolation of canola protein
Ser W Y, et al.
Food Sci. Technol., 41(5), 934-941 (2008)
Glucosinolate enhancement in cabbage induced by jasmonic acid application.
Fritz, V. A., Justen, V. L., Bode, A. M., Schuster, T., & Wang, M.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 45, 1188-1191 (2010)
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Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Questions

1–5 of 5 Questions  

  1. Can Sulfatase S9626 be dissolved in 0.2% NaCl?

    1 answer

    1. Solubility in 0.2% NaCl has not been tested for this product.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. How is Product S9626, Sulfatase from helix pomatia, prepared?

    1 answer

    1. This enzyme is isolated by acetone fractionation.

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  4. What is the solubility of Product S9626, Sulfatase from Helix pomatia?

    1 answer

    1. Prior to determining the activity of this enzyme, Sigma reconstitutes it in 0.2% (w/v) sodium chloride.

      Helpful?

  5. Does Product S9626, Sufatase from Helix pomatia, contain B-Glucuronidase activity?

    1 answer

    1. This product contains >300 units/mg solid B-Glucuronidase activity.

      Helpful?

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