S0198

Monoclonal Anti-Sonic Hedgehog peptide (N-terminal) antibody produced in rat

clone 171001, purified immunoglobulin, lyophilized powder

• Synonym(s): Anti-Dsh, Anti-Hhg1, Anti-Hx, Anti-Shh
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 171001, monoclonal
• form: lyophilized powder
• species reactivity: mouse, human
• technique(s): western blot: 1-2 μg/mL
• isotype: IgG2a
• UniProt accession no.: Q15465
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SHH(6469) ; mouse ... Shh(20423)

Description

Immunogen

purified, E. coli-derived recombinant mouse 6× histidine-tagged sonic hedgehog N-terminal peptide (aa 25-198).

Biochem/physiol Actions

Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, gamma catenin, myc and other transcription factors. The Hh family members have multiple roles such as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during the development of brain and nervous system.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves