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R9782

Sigma-Aldrich

RS-1

≥98% (HPLC)

Synonym(s):

3-[(benzylamino)sulfonyl]-4-bromo-N-(4-bromophenyl)benzamide, 4-Bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]-benzamide, RAD51-stimulatory compound 1

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About This Item

Empirical Formula (Hill Notation):
C20H16Br2N2O3S
CAS Number:
312756-74-4
Molecular Weight:
524.23
MDL number:
MFCD00348720
UNSPSC Code:
12352200
PubChem Substance ID:
329824048
NACRES:
NA.77

Assay

≥98% (HPLC)

form

powder

color

off-white to light tan

solubility

DMSO: ≥10 mg/mL

storage temp.

room temp

SMILES string

Brc1ccc(NC(=O)c2ccc(Br)c(c2)S(=O)(=O)NCc3ccccc3)cc1

InChI

1S/C20H16Br2N2O3S/c21-16-7-9-17(10-8-16)24-20(25)15-6-11-18(22)19(12-15)28(26,27)23-13-14-4-2-1-3-5-14/h1-12,23H,13H2,(H,24,25)

InChI key

SWKAVEUTKGKHSR-UHFFFAOYSA-N

Application

RS-1 has been shown to enhance CRISPR genome editing efficiency. To see other small molecule CRISPR enhancers, visit sigma.com/CRISPR-enhancers.
RS-1 has been used:
  • as a homology-directed repair (HDR) agonist to study its effects on in human hematopoietic stem/progenitor cells (HSPCs)
  • as HDR agonist to analyze its effects on DNA repair modulation in human induced pluripotent stem (iPS) cells
  • as RAD51 agonist to study its effects on double-stranded break repair

Biochem/physiol Actions

RS-1 (RAD51-stimulatory compound 1) is a stimulator of the human homologous recombination (HR) protein RAD51. RS-1 stimulates binding of hRAD51 to single stranded DNA (ssDNA) and enhances recombinogenic activity by stabilizing the active form of hRAD51 filaments without inhibiting hRAD51 ATPase activity. RS-1 has been shown to enhance CRISPR-Cas9 knock-in efficiency in HEK293A cells and has been shown to enhance both TALEN and Cas9-mediated knock-in efficiency in rabbits.
RS-1 is a sulfonamido-benzamide compound.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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