c-K-Ras or KRAS (kirsten rat sarcoma-2 virus oncogene) is a small GTP-binding protein. It is located on human chromosome 12p12.1. KRAS has two splice variants, such as KRASA and KRASB (KRAS proto-oncogenes). Expression of KRASA is restricted to specific tissues whereas KRASB is expressed everywhere .
Specificity
The antibody reacts with c-K-Ras and v-K-Ras p21. It crossreacts with cH-Ras and cN-Ras p21 on immunoblots.
Immunogen
recombinant p21 protein
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper)
Biochem/physiol Actions
KRAS (kirsten rat sarcoma-2 virus oncogene) mutation leads to poor prognosis of early rectal cancer.
Physical form
Solution in 0.05 M sodium phosphate buffer, pH7.5, containing 0.2% gelatin and < 0.1% sodium azide
Preparation Note
Purified using protein A or G
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Molecular cancer therapeutics, 12(12), 2847-2856 (2013-10-01)
Metformin is an oral biguanide commonly used for the treatment of type II diabetes and has recently been demonstrated to possess antiproliferative properties that can be exploited for the prevention and treatment of a variety of cancers. The mechanisms underlying
The Biochemical journal, 426(1), 65-72 (2009-11-20)
Oncogenic Ras mutations render the protein constitutively active and promote tumorigenesis via chronic stimulation of effector pathways. In A549 lung adenocarcinoma approx. 50% of the total Ras population is constitutively active, yet these cells display only weak activation of the
Although the protooncogenes small GTPases Ras are redox-sensitive proteins, how they are regulated by redox signaling in the central nervous system (CNS) is still poorly understood. Alteration in redox-sensitive targets by redox signaling may have myriad effects on Ras stability
Proceedings of the National Academy of Sciences of the United States of America, 110(25), 10201-10206 (2013-06-06)
Aberrant signaling by oncogenic mutant rat sarcoma (Ras) proteins occurs in ∼15% of all human tumors, yet direct inhibition of Ras by small molecules has remained elusive. Recently, several small-molecule ligands have been discovered that directly bind Ras and inhibit
The Journal of biological chemistry, 287(52), 43573-43584 (2012-11-06)
Oncogenic mutant Ras is frequently expressed in human cancers, but no anti-Ras drugs have been developed. Since membrane association is essential for Ras biological activity, we developed a high content assay for inhibitors of Ras plasma membrane localization. We discovered
Quantitative and qualitative western blotting to validate knockdown by esiRNA.
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