P5810

Monoclonal Anti-CD3−PE antibody produced in mouse

clone UCHT-1, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-CD3
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: UCHT-1, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): flow cytometry: 10 μL using 1 × 10⁶ cells
• isotype: IgG1
• UniProt accession no.: P07766
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD3E(916)

Description

General description

Mouse monoclonal clone UCHT-1 PE conjugated anti-Human CD3 recognizes the CD3 complex which is composed of 5 chains designated g, d, e, z and h having a molecular mass distribution of 16, 20, and 25 - 28 kDa. The antibody stains the cytoplasm of cerebellar Purkinje cells but does not stain B lymphocytes, monocytes, granulocytes, or NK cells. The epitope recognized by clone UCHT-1 is expressed on the e-chain of the CD3 antigen/T cell receptor complex. Detection of the epitope appears to be dependent of the binding to CD3-g or CD3-s. The epitope is sensitive to routine formalin fixation and paraffin embedding. Cryostat sections post-fixed in formalin can also be stained.

The CD3 human lymphocyte surface antigen is a glycoprotein thought to be associated with the T cell antigen receptor and to be involved in transmission of activation signals. The CD3 antigen is present on 60-80% of normal peripheral blood mononuclear cells, 20-40% of normal spleen cells, 40% of normal thymocytes, the majority of T-CLL and approximately 70% of T-ALL.

Specificity

Recognizes the CD3 complex. The epitope recognized by clone no. UCHT-1 is expressed on the ε-chain of the CD3 antigen/T cell receptor complex. 3rd Workshop: code no. 126, 208 and 471
4th Workshop: paper no. T3.2

Immunogen

human thymocytes followed by Sezary T cells.

Application

Mouse monoclonal clone UCHT-1 PE conjugated anti-Human CD3 may be used for: enumeration of total T lymphocytes in bone marrow, blood, and other body fluids; identification and localization of normal and malignant T lymphocytes, lymphoid, and other tissues; identification of leukemias and lymphomas of T cell origin and T lymphocyte activation studies

Target description

The CD3 complex (16/20/29/22/25-28 kDa) consists of 5 polypeptide chains associated with each other and with the T cell receptor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to R-phycoerythrin (PE). This orange-red dye is efficiently excited at 488 nm and emits at 578 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves