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P4109

Sigma-Aldrich

β-Phosphoglucomutase from Lactococcus sp.

recombinant, expressed in E. coli

Synonym(s):

β-D-Glucose 1,6-phosphomutase

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250 UNITS
£137.00
1000 UNITS
£465.00

£137.00


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250 UNITS
£137.00
1000 UNITS
£465.00

About This Item

Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

£137.00


Please contact Customer Service for Availability

recombinant

expressed in E. coli

form

solid

specific activity

≥10 unit/mg solid

storage temp.

−20°C

Related Categories

Application

β-Phosphoglucomutase is used to study starch and sucrose metabolism[1]. It is used for the determination of α-amylase in clinical analysis. It is used to study galactose utilization[2] and generalized glycogenosis (Pompe′s Disease)[3].

Biochem/physiol Actions

β-Phosphoglucomutase enzymatically converts β-D-glucose 1-phosphate to β-D-glucose 6-phosphate. It is involved in starch and sucrose metabolism[1].
Enzymatically converts β-D-glucose-1-phosphate to β-D-glucose-6-phosphate.

Unit Definition

One unit is defined as the amount of enzyme which converts 1μmol of β-D-glucose-1-phosphate to β-D-glucose-6-phosphate per minute at 37°C and pH 7.0.

Other Notes

Contains EDTA and lactose.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Erika Pellegrini et al.
Acta crystallographica. Section D, Biological crystallography, 67(Pt 10), 902-906 (2011-09-21)
Over the last 20 years cryocrystallography has revolutionized the field of macromolecular crystallography, greatly reducing radiation damage and allowing the collection of complete data sets at synchrotron sources. However, in order to cool crystals to 100 K cryoprotective agents must usually
Jianying Dai et al.
Biochemistry, 45(25), 7818-7824 (2006-06-21)
Activated Lactococcus lactis beta-phosphoglucomutase (betaPGM) catalyzes the conversion of beta-d-glucose 1-phosphate (betaG1P) derived from maltose to beta-d-glucose 6-phosphate (G6P). Activation requires Mg(2+) binding and phosphorylation of the active site residue Asp8. Initial velocity techniques were used to define the steady-state
OCULAR HISTOPATHOLOGY IN GENERALIZED GLYCOGENOSIS (POMEPE'S DISEASE).
D TOUSSAINT et al.
Archives of ophthalmology (Chicago, Ill. : 1960), 73, 342-349 (1965-03-01)
A phosphoglucomutase specific for β-glucose 1-phosphate
Ben-Zvi R and Schramm M
The Journal of Biological Chemistry, 236, 2186-2189 (1961)
G G Hardy et al.
Infection and immunity, 69(4), 2309-2317 (2001-03-20)
Synthesis of the Streptococcus pneumoniae type 3 capsule requires the pathway glucose-6-phosphate (Glc-6-P) --> Glc-1-P --> UDP-Glc --> UDP-glucuronic acid (UDP-GlcUA) --> (GlcUA-Glc)(n). The UDP-Glc dehydrogenase and synthase necessary for the latter two steps, and essential for capsule production, are

Questions

  1. Hello, I received the enzyme pgmB (P4109) and wondered in what buffer should I dissolve the enzyme powder. Thank you

    1 answer
    1. This product may be reconstituted in 10 mM Potassium Phosphate, pH 7 at 5mg/mL. The stock solution is expected to be stabe for at least 1 week when stored refrigerated. For assay purposes, the enzyme is prepared just prior to use with ice cold 10 mM Potassium Phosphate, pH 7 solution duluted to 1.0 - 3.0 U/mL.

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