P0157
Phosphatase, Acid from potato
ammonium sulfate suspension, ≥200 units/mg protein (biuret)
Synonym(s):
(Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato
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About This Item
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form
ammonium sulfate suspension
specific activity
≥200 units/mg protein (biuret)
mol wt
111 kDa
foreign activity
Apyrase (ATPase) ≤5% of base activity
storage temp.
2-8°C
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
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General description
Acid phosphatase from potatoes is a 111 kDa diner consisting of two subunits at 41 and 35 kDa. This phosphatase has also been shown to cleave DNA.
Application
Phosphatase acid from potato has been used in a study to develop a method of efficient enzymatic hydrolysis of polyprenyl pyrophosphates. It has also been used in a study to investigate the kinetics of the hydrolysis of sodium p-nitrophenylphosphate and other phosphoric acid monoesters.
Biochem/physiol Actions
Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.
Physical form
Suspension in 1.8 M (NH4)2SO4, 10 mM MgCl2, pH 5.5
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Y Mikuni-Takagaki et al.
The Biochemical journal, 268(3), 585-591 (1990-06-15)
In order to understand the mechanism of the post-translational processing of bone phosphoproteins in embryonic bone, periosteal bone strips isolated from 12-day-embryonic-chick tibiae were cultured and the bone proteins labelled with Na2H32PO4. Of the total radiolabelled proteins recovered from the
Insight into bio-physiological functions of acid phosphatase from potatoes (Solanum tuberosum): A bioanalytical approach.
Rane, S.
Indian Journal of Chemistry, 48a, 15-24 (2009)
The kinetics and mechanism of the hydrolysis of phosphoric acid esters by potato acid phosphatase.
E F ALVAREZ
Biochimica et biophysica acta, 59, 663-672 (1962-06-04)
H Fujii et al.
Biochimica et biophysica acta, 712(3), 716-718 (1982-09-14)
A method of efficient enzymatic hydrolysis of polyprenyl pyrophosphates was established. Polyprenyl pyrophosphates such as all-trans-octaprenyl and all-trans-nonaprenyl (solanesyl) pyrophosphate can be hydrolyzed completely to the corresponding primary alcohols by the action of potato acid phosphatase in the presence of
S I Tu et al.
Plant physiology, 88(1), 61-68 (1988-09-01)
Primary cell walls, free from cytoplasmic contamination were prepared from corn (Zea mays L.) roots and potato (Solanum tuberosum) tubers. After EDTA treatment, the bound acid phosphatase activities were measured in the presence of various multivalent cations. Under the conditions
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