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N4637

Sigma-Aldrich

Newborn Calf Serum

USA origin, sterile-filtered, suitable for cell culture

Synonym(s):

NBCS, NBS, NCS, calf sera, calf serum, newborn calf sera

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About This Item

MDL number:
MFCD00163621
UNSPSC Code:
12352207
NACRES:
NA.71

biological source

bovine (calf)

sterility

sterile-filtered

form

liquid

composition

hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤100 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

Newborn calf serum (NCS) is collected within 10 days post partum. NCS is useful in a wide range of cell culture and organ preservation applications. For example, it is used in cornea preservation and cryopreservation of bovine embryos. NCS is used in cell culture to support the maturation of oocytes; to study the function of tubular epithelial cells; and to develop primary cultures of cells such as visceral adipocytes.

Preparation Note

Collected from calves that are 10 days old or less.

Analysis Note

Endotoxin tested

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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To establish a defined in vitro maturation culture system for porcine oocytes, we examined the effects of adding cysteine (Cys) and epidermal growth factor (EGF) to the maturation medium. Furthermore, to evaluate cytoplasmic maturation, we investigated GSH concentrations and embryo
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