Nile Blue A is available in various forms or salts, such as sulfate, perchlorate, or chloride. Suggestion is to use the chloride form in a similar manner to the sulfate form and make adjustments based on the staining results.
N0766
Nile Blue A
Dye content, ≥75%, certified by the Biological Stain Commission, powder
Synonym(s):
Basic Blue 12, Nile blue sulfate
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5 G
£74.30
25 G
£274.00
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5 G
£74.30
25 G
£274.00
About This Item
Linear Formula:
2C20H20N3O · SO4
CAS Number:
Molecular Weight:
732.85
Colour Index Number:
51180
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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Product Name
Nile Blue A, certified by the BSC
Agency
certified by the BSC
form
powder
composition
Dye content, ≥75%
technique(s)
microbe id | staining: suitable
color
dark green
mp
>300 °C (dec.) (lit.)
solubility
H2O: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[O-]S([O-])(=O)=O.CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34.CC\[N+](CC)=C5/C=CC6=Nc7c(OC6=C5)cc(N)c8ccccc78
InChI
1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)
InChI key
QIRDPEPUXNCOLD-UHFFFAOYSA-N
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Application
Features and Benefits
Used in the preparation of an amperometric glucose sensor.
Suitability
Certified for use in Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue; Lillie and Fuller method for fatty acids/lipids using frozen sections of fatty liver and avocado.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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