MDQ1
Imprint® Methylated DNA Quantification Kit
To measure global DNA methylation shifts from as low as 10 ng DNA
Synonym(s):
DNA Methylation Quantification, DNA methylation shift
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.54
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General description
Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.
Application
Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.
Features and Benefits
- ELISA based format
- Procedure completes in 4 easy to follow steps
- All reagents supplied including control methylated DNA
- Detection limit is 5 ng of fully methylated DNA
- Input DNA may be as low as 10-200 ng
- Procedure completes in 3.5 hours
- 96 well format allows option of studying single samples or high-throughput studies
- Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
- May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit Components Also Available Separately
Product No.
Description
SDS
- M8570Methylated Control DNA, 50 ng/μLSDS
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Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
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Articles
Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.
Protocols
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
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