M3671

MES

low moisture content, ≥99% (titration)

• Synonym(s): 2-(N-Morpholino)ethanesulfonic acid, 4-Morpholineethanesulfonic acid
• Empirical Formula (Hill Notation): C₆H₁₃NO₄S
• CAS Number: 4432-31-9
• Molecular Weight: 195.24
• Beilstein: 141319
• EC Number: 224-632-3
• MDL number: MFCD00006181
• UNSPSC Code: 12161700
• eCl@ss: 32129211
• PubChem Substance ID: 24896867
• NACRES: NA.25

Properties

• Quality Level: 400
• Assay: ≥99% (titration)
• form: powder
• storage condition: dry at room temperature
• impurities: ≤1.0% water
• color: white
• pH: 2.5-4 (25 °C, 97.6 g/L)
• useful pH range: 5.5-6.7
• pKa: 6.1
• density: 1.23 g/cm³ at 20—25 °C; 1.27 g/cm³ at 20—25 °C
• application(s): clinical research; diagnostic assay manufacturing; life science and biopharma (; ); metabolomics (; )
• SMILES string: OS(=O)(=O)CCN1CCOCC1
• InChI: 1S/C6H13NO4S/c8-12(9,10)6-3-7-1-4-11-5-2-7/h1-6H2,(H,8,9,10)
• InChI key: SXGZJKUKBWWHRA-UHFFFAOYSA-N

Description

General description

MES Buffer, recognized as one of the "Good" buffers employed in biological and biochemical applications, plays a crucial role across various research domains. This zwitterionic and morpholinic buffer proves highly valuable due to its effective buffering capacity within the pH range of 5.5 to 6.7. Notably, MES Buffer does not engage in complex formation with the majority of metals commonly employed in environmental and biological studies. Its solubility in water is excellent, and it exhibits minimal lipid solubility, rendering it impermeable to membranes.

This versatile buffer may find widespread application in maintaining a stable environment in solutions dedicated to cell culture media, protein-based buffer formulations, and electrophoresis running buffers. Its significance extends to the purification processes of antibodies, peptides, proteins, blood components, and growth factors. MES Buffer stands out as an optimal choice for capillary electrochromatography, offering low ionic mobility. Notably, it serves as a safer alternative to potentially toxic agents like cacodylate and non-zwitterionic buffers, including citrate and malate. MES Buffer also proves valuable for fine-tuning the pH of growth media, stabilizing enzymatic solutions, and serving as a component of PAGE running buffer in diverse experimental settings.

Application

It has been utilized as a buffer bicarbonate‐containing culture medium[1]

MES has been used as a component of the elution buffer used to elute plasma from α2-macroglobulin affinity column.[2] It has been used as an activation buffer during antibody conjugation to microparticles.[3] MES has been used in the functionalization of microchannels during the creation of microfluidic-integrated surface plasmon resonance (SPR) platform for pathogen detection[4].

Features and Benefits

• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)
• Highly soluble in water
• Minimal metal ion binding
• Less toxic to cells than other buffers such as Tris and phosphate
• Stable in a wide pH range
• Low UV absorptivity and Minimal reactivity

Quality

Low moisture

Other Notes

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Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves