Recommended Products
type
for DNA synthesis
product line
Proligo Reagents
pore size
500 Å
λ
conforms (UV/VIS Identity)
storage temp.
−20°C
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General description
Amino-ON CPG can be employed to introduce a covalently bound, primary amino group at the 3′-end of an oligonucleotide. It comprises a protected hexylamino group. The free amine is liberated under the alkaline conditions that are employed to cleave and deprotect the oligonucleotide on the support, while the hexylamino group remains attached to the oligonucleotide. The Amino-ON CPG further comprises a DMT-protective group which is removed in the first synthesis cycle. The synthesis of oligonucleotides is conducted in the same manner as with nucleoside-loaded CPG, without changes in any of the reagents of the synthesis. Amino-ON CPG can be employed to conjugate biotin, fluorescein or other modifiers and reporter groups to the 3′-end of oligonucleotides or to attach oligonucleotides to surfaces. Proligo′s Amino-ON CPG combines the advanced features of cleavage and deprotection under mild conditions, and maintenance of the integrity of the 3′-modification with high synthesis yields.
Features and Benefits
- Introduces a primary amino group attached to a C6-linker
- Fully compatible with standard phosphoramidite reagents and synthesis conditions
- Can be used with DNA and RNA; 5′-oligonucleotide modifications such as fluorescein or biotin; and with the synthesis of thioated DNA oligonucleotides
- Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotide can be cleaved from the support and deprotected in concentrated ammonia at 55 °C in 2 hours
- Provides all the advantages of homogeneous pore-size porous glass supports
- DMT-protected in the same way as standard nucleoside-loaded CPG
Legal Information
This product and it use is covered under US Patent Number 7,329,515
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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