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KEM0031

Sigma-Aldrich

Klenow Fragment

Ultra-pure enzyme for nucleic acid modifications

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About This Item

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grade

for molecular biology

Assay

>99% (SDS-PAGE)

form

buffered aqueous solution

specific activity

5,000 U/mg

concentration

5,000 U/mL

shipped in

dry ice

storage temp.

−20°C

General description

Klenow Fragment is a mesophilic DNA polymerase derived from the E.coli Polymerase I DNA-dependent repair enzyme. The enzyme exhibits DNA synthesis and proofreading (3′ → 5′) nuclease activities, and, in the absence of the holoenzyme′s (5′→3′) nuclease domain, displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene.

Application

Suitable for:
  • DNA blunting by fill-in of 5′ overhang
  • Second strand cDNA synthesis
  • Sequencing
  • Site-specific mutagenesis

Features and Benefits

  • Ultra-purification process for ultimate enzyme performance
  • Highest quality specifications for ultimate product consistency
  • Undetectable DNA and nuclease contamination

Components

Supplied with:KEM0042B (10X Blue Buffer)

Unit Definition

1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37° C.

Physical form

Supplied in 100 mM KPO4, 1.0 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 7.4 @ 25° C.

Other Notes

Source of protein: A recombinant E. coli strain carrying the Klenow Fragment gene.
Unit size: 2,500 U

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

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Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing

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