publication info
A. Bates, et al., Springer-Verlag, 2000, 346 pp., soft cover
manufacturer/tradename
Garland
General description
This second edition provides a structured approach to learning by covering all the important topics in a uniform, systematic format. Each topic begins with a summary of all essential facts, an ideal revision checklist, followed by detailed explanation and clear, simple diagrams. Topics covered include cells and macromolecules, protein structure, properties of nucleic acids, prokaryotic and eukaryotic chromosome structure, and DNA replication.
Table of Contents
CELLS AND MACROMOLECULES
Cellular classification
Subcellular organelles
Macromolecules
Large macromolecular assemblies
PROTEIN STRUCTURE
Amino acids
Protein structure and function
Protein analysis
PROPERTIES OF NUCLIEC ACIDS
Nucleic acid structure
Chemical and physical properties of nucleic acids
Spectroscopic and thermal properties of nucleic acids
DNA supercoiling
PROKARYOTIC AND EUKARYOTIC CHROMOSOME STRUCTURE
Prokaryotic chromosome structure
Chromatin structure
Eukaryotic chromosome structure
Genome complexity
The flow of genetic information
DNA REPLICATION
DNA replication: an overview
Bacterial DNA replication
The cell cycle
Eukaryotic DNA replication
DNA DAMAGE, REPAIR AND RECOMBINATION
Mutagenesis
DNA damage
DNA repair
Recombination
GENE MANIPULATION
DNA cloning: an overview
Preparation of plasmid DNA
Restriction enzymes and electrophoresis
Ligation, transformation and analysis of recombinants
CLONING VECTORS
Design of plasmid vectors
Bacteriophage vectors
Cosmids, YACs and BACs
Eukaryotic vectors
GENE LIBRARIES AND SCREENING
Genomic libraries
cDNA libraries
Screening procedures
ANALYSIS AND USES OF CLONED DNA
Characterization of clones
Nucleic acid sequencing
Polymerase chain reaction
Organization of cloned genes
Mutagenesis of cloned genes
Applications of cloning
TRANSCRIPTION IN PROKARYOTES
Basic principles of transcription
Escherichia coli RNA polymerase
The E.coli promoter
Transcription, initiation, elongation and termination
REGULATION OF TRANSCRIPTION IN PROKARYOTES
The lac operon
The trp operon
Transcriptional regulation by alternative factors
TRANSCRIPTION IN EUKARYOTES
The three RNA polymerases: characterization and function
RNA pol I genes: the ribosomal repeat
RNA pol III genes: 5S and tRNA transcription
RNA pol II genes: promoters and enhancers
General transcription factors and RNA Pol II initiation
REGULATION OF TRANSCRIPTION IN EUKARYOTES
Eukaryotic transcription factors
Examples of transcriptional regulation
RNA PROCESSING AND RNPs
rRNA processing and ribosomes
tRNA processing, RNase P and ribozymes
mRNA processing, hnRNPs and snRNPs
Alternative mRNA processing
THE GENETIC CODE AND tRNA
The genetic code
tRNA structure and function
PROTEIN SYNTHESIS
Aspects of protein synthesis
Mechanisms of protein synthesis
Initiation in eukaryotes
Translational control and post-translational events
BACTERIOPHAGES AND EUKARYOTIC VIRUSES
Introduction to viruses
Bacteriophages
DNA viruses
RNA viruses
TUMOR VIRUSES AND ONCOGENES
Oncogenes found in tumor viruses
Categories of oncogenes
Tumor suppressor genes
Apoptosis
FURTHER READING
MULTIPLE CHOICE QUESTIONS
ANSWERS
Cellular classification
Subcellular organelles
Macromolecules
Large macromolecular assemblies
PROTEIN STRUCTURE
Amino acids
Protein structure and function
Protein analysis
PROPERTIES OF NUCLIEC ACIDS
Nucleic acid structure
Chemical and physical properties of nucleic acids
Spectroscopic and thermal properties of nucleic acids
DNA supercoiling
PROKARYOTIC AND EUKARYOTIC CHROMOSOME STRUCTURE
Prokaryotic chromosome structure
Chromatin structure
Eukaryotic chromosome structure
Genome complexity
The flow of genetic information
DNA REPLICATION
DNA replication: an overview
Bacterial DNA replication
The cell cycle
Eukaryotic DNA replication
DNA DAMAGE, REPAIR AND RECOMBINATION
Mutagenesis
DNA damage
DNA repair
Recombination
GENE MANIPULATION
DNA cloning: an overview
Preparation of plasmid DNA
Restriction enzymes and electrophoresis
Ligation, transformation and analysis of recombinants
CLONING VECTORS
Design of plasmid vectors
Bacteriophage vectors
Cosmids, YACs and BACs
Eukaryotic vectors
GENE LIBRARIES AND SCREENING
Genomic libraries
cDNA libraries
Screening procedures
ANALYSIS AND USES OF CLONED DNA
Characterization of clones
Nucleic acid sequencing
Polymerase chain reaction
Organization of cloned genes
Mutagenesis of cloned genes
Applications of cloning
TRANSCRIPTION IN PROKARYOTES
Basic principles of transcription
Escherichia coli RNA polymerase
The E.coli promoter
Transcription, initiation, elongation and termination
REGULATION OF TRANSCRIPTION IN PROKARYOTES
The lac operon
The trp operon
Transcriptional regulation by alternative factors
TRANSCRIPTION IN EUKARYOTES
The three RNA polymerases: characterization and function
RNA pol I genes: the ribosomal repeat
RNA pol III genes: 5S and tRNA transcription
RNA pol II genes: promoters and enhancers
General transcription factors and RNA Pol II initiation
REGULATION OF TRANSCRIPTION IN EUKARYOTES
Eukaryotic transcription factors
Examples of transcriptional regulation
RNA PROCESSING AND RNPs
rRNA processing and ribosomes
tRNA processing, RNase P and ribozymes
mRNA processing, hnRNPs and snRNPs
Alternative mRNA processing
THE GENETIC CODE AND tRNA
The genetic code
tRNA structure and function
PROTEIN SYNTHESIS
Aspects of protein synthesis
Mechanisms of protein synthesis
Initiation in eukaryotes
Translational control and post-translational events
BACTERIOPHAGES AND EUKARYOTIC VIRUSES
Introduction to viruses
Bacteriophages
DNA viruses
RNA viruses
TUMOR VIRUSES AND ONCOGENES
Oncogenes found in tumor viruses
Categories of oncogenes
Tumor suppressor genes
Apoptosis
FURTHER READING
MULTIPLE CHOICE QUESTIONS
ANSWERS
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service