CHP2
Imprint® Ultra Chromatin Immunoprecipitation Kit
Complete ChIP kit for sensitivity, compatibility with Next-Gen sequencing
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About This Item
UNSPSC Code:
12352202
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General description
The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 106 cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 108 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.
Application
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Features and Benefits
- Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
- Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 106
- Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 106 cells
- Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit Components Only
Product No.
Description
- Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water
- Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution
Kit Components Also Available Separately
Product No.
Description
SDS
- S6576ChIP Next Gen Seq Sepharose™SDS
- I8896IGEPAL® CA-630, for molecular biologySDS
- S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell cultureSDS
- W4502Water, Nuclease-Free Water, for Molecular BiologySDS
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderSDS
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderSDS
- A7638Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)SDS
- A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)SDS
- D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridizationSDS
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionSDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS
- R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)SDS
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1A - STOT SE 3
Target Organs
Central nervous system
Storage Class Code
5.1A - Strongly oxidizing hazardous materials
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Protocols
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
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