Lu2
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 99043003-DNA-5UG
pkg of vial of cells 99043003-1VL
ImmunoDon
BALB/c x C57 Bl/6
Myeloma
P3x63Ag8.653
Reactivity
Anti-DR11
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse x Mouse hybridoma
Cell Line Description
The cell line Lu2 has been established by fusion of the myeloma P3x63Ag8.653 and spleen cells from BALB/c x C57Bl/6 female mice. Mice received a single i.p. injection with the B cell line LuMar expressing HLA A2.3/B18.5/C5/DR3,11.and B. pertussis three days before fusion. The cell line has been eradicated from mycoplasma using Cibrobay.
Immunogen
B cell line LuMar
Application
Immunofluorescence, cytotoxicity, immunoprecipitation
Culture Medium
RPMI 1640 + 2mM glutamine + 10% FBS
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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