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CV-1 Clone B5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514

biological source

African green monkey kidney

packaging

tube of 5 μg 90032601-DNA-5UG
pkg of vial of cells 90032601-1VL

growth mode

Adherent

karyotype

2n = 60, pseudodiploid

morphology

Fibroblast

receptors

Not specified

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Cell Line Origin

Monkey African Green kidney fibroblast

Cell Line Description

The cells have been selected for sensitivity to SV40.

Culture Medium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 2% Tricine (1M solution at pH7.4) + 10% FBS / FCS (F2442).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

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