Ca Ski

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87020501, human cervix (small bowel metastasis), Epithelial

• UNSPSC Code: 41106514

Properties

• biological source: human cervix (small bowel metastasis)
• packaging: tube of 5 μg 87020501-DNA-5UG; pkg of vial of cells 87020501-1VL
• growth mode: Adherent
• karyotype: Modal no. 78
• morphology: Epithelial
• products: Beta Human chorionic gonadotropin (beta-hCG)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian cervical epidermoid carcinoma

Cell Line Description

Derived from an epidermoid carcinoma of the cervix metastatic to the small bowel mesentery of a 40 year old Caucasian. Ca Ski cells secrete the β subunit of human chorionic gonadotropin (β-h CG), express tumour-associated antigen and G6PD type B. Ca Ski cells have been reported to contain an integrated human papilloma virus 16 genome (HPV-16 about 600 copies per cell) and HPV-18 related sequences.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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