Recommended Products
product name
HeLa B, 85060701
biological source
human cervix
growth mode
Adherent
karyotype
Aneuploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human cervix carcinoma
Cell Line Description
A derivative of HeLa, the first aneuploid, epithelial-like line derived from human tissue and maintained by serial subculture. Identified as a contaminant in many subsequently derived lines, due to poor culture techniques. Susceptible to poliovirus 1 and Ad 3.
Application
Tumourigenicity and virus studies: poliovirus 1 and Ad 3
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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