C70 cell line human
12022903, human colon, epithelial
Synonym(s):
C 70, C-70
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About This Item
UNSPSC Code:
41106514
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Product Name
C70 cell line human, 12022903
biological source
human colon
growth mode
adherent
morphology
epithelial
shipped in
dry ice
storage temp.
−196°C
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Cell Line Description
The C70 cell line was established from a 60-year old female patient with a moderately well differentiated adenocarcinoma of the sigmoid colon classified as Dukes′ stage B.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11,12
D5S818: 10
D7S820: 9
THO1: 6,9.3
TPOX: 8,11
vWA: 17,18
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11,12
D5S818: 10
D7S820: 9
THO1: 6,9.3
TPOX: 8,11
vWA: 17,18
Culture Medium
Iscove′s Modified Dulbecco′s Medium, + 10% Fetal Bovine Serum (FBS) + 2 mM Glutamine
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.
C70 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
C70 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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