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recombinant
expressed in Streptomyces lividans
form
lyophilized solid
specific activity
≥18 unit/mg solid
mol wt
~37 kDa by SDS-PAGE
storage temp.
−20°C
Application
The enzyme sequence is derived from Nocardia sp., and this cholesterol dehydrogenase is expressed in Streptomyces lividans. Cholesterol Dehydrogenase from Nocardia sp. has been used in the preparation of amperometric cholesterol biosensor for cholesterol detection in biological samples and in a study to investigate the atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain with the LDL-receptor tail. Cholesterol dehydrogenase from Nocardia sp. Has also been used in a study to assess organization, dynamics, and segregation of Ras nanoclusters in membrane domains.
Enzyme used in determining cholesterol in serum
Biochem/physiol Actions
Cholesterol dehydrogenase (CDH) is a nicotinamide adenine dinucleotide phosphate (NAD(P)) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Cholesterol detection by using CDH allows direct and easier quantification compared to cholesterol oxidase based assay.
Cholesterol dehydrogenase is a NAD(P) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Molecular mass of the protein is found to be 37 kDa based on SDS-PAGE. Its pH is 4.5.
Unit Definition
One unit produces 1.0 μmole of cholesten-4-en-3-one per minute at pH 8.5 at 25 °C.
Physical form
Supplied as a lyophilized powder
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Proceedings of the National Academy of Sciences of the United States of America, 109(21), 8097-8102 (2012-05-09)
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A disposable amperometric biosensor for determining total cholesterol in whole blood
Fang C, et al.
Sensors and Actuators B, Chemical, 155(2) (2011)
The characteristics and applications of recombinant cholesterol dehydrogenase
Kishi K, et al.
Bioscience, Biotechnology, and Biochemistry, 64(7) (2000)
Cloning, nucleotide sequence, and transcriptional analysis of the NAD (P)-dependent cholesterol dehydrogenase gene from a Nocardia sp. and its hyperexpression in Streptomyces spp.
Horinouchi S, et al.
Applied and Environmental Microbiology, 57(5) (1991)
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