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Key Documents

B4657

Sigma-Aldrich

5-Bromo-6-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt

≥98%

Synonym(s):

Magenta-GlcA, Magenta glucuronide

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About This Item

Empirical Formula (Hill Notation):
C14H13BrClNO7 · C6H13N
CAS Number:
Molecular Weight:
521.79
MDL number:
UNSPSC Code:
12352200

Assay

≥98%

storage temp.

−20°C

SMILES string

NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4cc(Cl)c(Br)cc34

InChI

1S/C14H13BrClNO7.C6H13N/c15-5-1-4-7(2-6(5)16)17-3-8(4)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1

InChI key

HGZDFBMYVMPFHR-CYRSAHDMSA-N

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Application

Chromogenic substrate for β-glucuronidase; produces a magenta color in GUS+ bacterial colonies.

Linkage

An alternative to 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X−GlcA) for detection of the β-glucuronidase gene.

Legal Information

Magenta is a trademark of Merck KGaA, Darmstadt, Germany

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E. coli beta-glucuronidase (GUS) as a marker for recombinant vaccinia viruses.
M W Carroll et al.
BioTechniques, 19(3), 352-354 (1995-09-01)

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