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Key Documents

B4500

Sigma-Aldrich

Nα-Benzoyl-L-arginine ethyl ester hydrochloride

trypsin substrate, chromogenic, ≥97% (HPLC), powder

Synonym(s):

BAEE

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About This Item

Empirical Formula (Hill Notation):
C15H22N4O3 · HCl
CAS Number:
Molecular Weight:
342.82
Beilstein:
3781694
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

product name

Nα-Benzoyl-L-arginine ethyl ester hydrochloride, trypsin substrate

Quality Level

Assay

≥97% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless

storage temp.

2-8°C

SMILES string

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1

InChI key

HIXDELXKSSLIKB-YDALLXLXSA-N

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General description

Nα-Benzoyl-L-arginine ethyl ester hydrochloride is a substrate for trypsin. It is a nonprotein substrate used in spectroscopic assays.

Application

  • N

  • α-Benzoyl-L-arginine ethyl ester hydrochloride has been used as a substrate:
  • in peptidyl arginine deiminase (PPAD) assay in P. gingivalis vesicles
  • for assaying proteolytic activity of enzymatic extracts of papaya plant parts
  • in trypsin activity assay of the tail tendon fascicles from rat

Substrates

The prototype substrate for trypsin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Optima of Trypsin-Catalyzed Hydrolysis and Its Inhibition Determined by SDS-PAGE
Zhou X, et al.
Advances in Enzyme Research, 4(01), 1-1 (2016)
Proteolytic activity in enzymatic extracts from Carica papaya L. cv. Maradol harvest by-products
Thomas GE, et al.
Process Biochemistry (Oxford, United Kingdom), 44(1), 77-82 (2009)
The effect of triclosan on posttranslational modification of proteins through citrullination and carbamylation
Bright R, et al.
Clinical Oral Investigations, 22(1), 487-493 (2018)
Maria T Dulay et al.
Analytical chemistry, 77(14), 4604-4610 (2005-07-15)
Trypsin is covalently linked to a photopolymerized sol-gel monolith modified by incorporating poly(ethylene glycol) (PSG-PEG) for on-column digestion of N(alpha)-benzoyl-l-arginine ethyl ester (BAEE) and two peptides, neurotensin and insulin chain B. The coupling of the enzyme to the monolith is
Mohammad K Hossain et al.
Colloids and surfaces. B, Biointerfaces, 181, 85-93 (2019-05-28)
Electric current responsive magnetic composite particles are prepared in three steps. In the first step, spherical and mesoporous submicrometer-sized magnetic iron oxide (Fe3O4) core particles are prepared by solvothermal method. Then magnetic Fe3O4 particles are functionalized with amine groups using

Articles

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Protocols

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

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