Nuclear factor, interleukin 3 regulated (NFIL3, E4BP4) is a transcription factor involved in development of haematopoietic lineages. NFIL3/E4BP4 is required for development of NK cells and CD8α(+) conventional dendritic cells, and myeloid lineage commitment. NFIL3/E4BP4 also facilitates macrophage activation, polarisation of CD4(+) T cell responses and B cell class switching to IgE.
Specificity
Anti-NFIL3 polyclonal antibody reacts with human, bovine, and canine nuclear factor, interleukin 3 regulated proteins.
Immunogen
Synthetic peptide directed towards the N terminal region of human NFIL3
Application
Anti-NFIL3 polyclonal antibody is used to tag nuclear factor, interleukin 3 regulated for detection and quantitation by Western blotting and in plasma by immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of nuclear factor, interleukin 3 regulated in haematopoietic lineages development and specialized immune function regulation.
Biochem/physiol Actions
NFIL3 is a basic leucine zipper (bZIP) transcription factor that represses or activates transcription in non-osteoblastic cells. NFIL3 play a role in attenuation of PTH target gene transcription in osteoblasts. NFIL3 repression domain interacts specifically with the TBP binding repressor protein Dr1.Glucocorticoid-mediated upregulation of the bZIP transcriptional repressor gene, NFIL3, is dependent on [Ca2+]i levels, and correlates with Glucocorticoid-evoked apoptosis of Glucocorticoid-sensitive CEM-C7-14 cells. NFIL3 is involved in a distinct growth factor-regulated signaling pathway that is responsible for the survival of early B-cell progenitors, and whose alteration by E2A-HLF leads to childhood B lineage leukemia.
Sequence
Synthetic peptide located within the following region: MQLRKMQTVKKEQASLDASSNVDKMMVLNSALTEVSEDSTTGEDVLLSEG
Physical form
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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