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A8427

Sigma-Aldrich

Antipyrylazo III

suitable for determination of calcium (per Anal. Chim. Acta, 71, 343 (1974)), ~95%

Synonym(s):

3,6-Bis(4-antipyrylazo)-4,5-dihydroxy-2,7-naphthalenedisulfonic acid disodium salt

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About This Item

Empirical Formula (Hill Notation):
C32H26N8Na2O10S2
CAS Number:
14918-39-9
Molecular Weight:
792.71
Beilstein:
956469
EC Number:
238-988-2
MDL number:
MFCD00042700
UNSPSC Code:
12352204

Assay

~95%

suitability

suitable for determination of calcium (per Anal. Chim. Acta, 71, 343 (1974))

SMILES string

[Na+].[Na+].CN1N(c2ccccc2)C(=O)C(\N=N\c3c(O)c4c(O)c(\N=N\C5=C(C)N(C)N(c6ccccc6)C5=O)c(cc4cc3S([O-])(=O)=O)S([O-])(=O)=O)=C1C

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Linkage

Similar to A 5894, but produced by Sigma.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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A V Tepikin et al.
The Journal of membrane biology, 123(1), 43-47 (1991-07-01)
Simultaneous optical measurements of extra- and intracellular Ca2+ concentrations were carried out on isolated snail neurons injected iontophoretically with Ca2+. The fluorescent indicator Fura-2 was used to measure intracellular concentration of free Ca, and the absorbant indicator Antipyrylazo III to
A Barth et al.
Biochimica et biophysica acta, 1057(1), 115-123 (1991-03-01)
Fourier transform infrared spectroscopy was used to investigate ligand binding and conformational changes in the Ca2(+)-ATPase of sarcoplasmic reticulum during the catalytic cycle. The ATPase reaction was started in the infrared sample by release of ATP from the inactive, photolabile
H Sun et al.
Biochimica et biophysica acta, 1403(1), 57-71 (1998-06-12)
The accurate measurement of the intracellular concentration of free magnesium ions ([Mg2+]i) is essential for evaluating the role of Mg2+ in cellular functions. Among the specific (compared to fluorescent indicators) metallochromic dyes, antipyrylazo III (APIII) appears to be most suitable
J Garcia et al.
The Journal of physiology, 463, 709-728 (1993-04-01)
1. Calcium transients were recorded from cut segments of fast-twitch rat skeletal muscle fibres stretched to 3.7-4.0 microns per sarcomere and voltage clamped at a holding potential of -80 mV using the double Vaseline-gap technique. Calcium transients were monitored simultaneously
B J Simon et al.
The Journal of general physiology, 97(3), 437-471 (1991-03-01)
The steady-state calcium dependence of inactivation of calcium release from the sarcoplasmic reticulum was studied in voltage-clamped, cut segments of frog skeletal muscle fibers containing two calcium indicators, fura-2 and anti-pyrylazo III (AP III). Fura-2 fluorescence was used to monitor
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