A2382
Azoalbumin
protease substrate
Synonym(s):
Sulfanilic acid-azoalbumin
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About This Item
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biological source
bovine
Quality Level
form
powder
concentration
60-90% (Lowry)
technique(s)
tissue culture: suitable
solubility
0.01 M HCl: soluble 25 mg/mL
storage temp.
2-8°C
General description
Azoalbumin is a substrate for assaying proteolytic enzymes, majorly trypsin, pepsin and cysteine proteinase. The assay method offers precision and is easy to perform.
Application
Azoalbumin has been used:
- as a substrate for enzymatic assay of hyphal extracts
- for screening general protease activity in spent growth medium extract
- for screening S. rolfsii proteases
Preparation Note
Prepared from Bovine albumin, Fraction V (A 4503)
Substrates
A soluble chromogenic substrate for proteolytic enzymes.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Journal of reproduction and fertility, 51(1), 65-71 (1977-09-01)
No unusual steroid-binding proteins that might react with the oocyte or its investments could be detected in follicular fluid. Corticosteroid-binding globulin occurred in follicular fluid from pigs, sheep and cows, and sex hormone-binding globulin occurred in follicular fluid from sheep
Optimisation of cellobiose dehydrogenase production by the fungus Sclerotium (Athelia) rolfsii
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A modified azoalbumin technique for the assay of proteolytic enzymes for use in blood group serology.
Journal of Clinical Pathology, 37(3), 329-331 (1984)
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