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P0532

Sigma-Aldrich

Phthaldialdehyde Reagent

Solution Complete

Synonym(s):

OPA

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.21

Quality Level

storage temp.

2-8°C

SMILES string

[H]C(=O)c1ccccc1C([H])=O

InChI

1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H

InChI key

ZWLUXSQADUDCSB-UHFFFAOYSA-N

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Application

Precolumn derivatization reagent for primary amines and amino acids. The fluorescent derivative can be detected by reverse-phase HPLC. The reaction requires OPA, primary amine and a sulfhydryl. In the presence of excess sulfhydryl, amines can be quantitated. In the presence of excess amine, sulfhydryls can be quantitated.

Other Notes

Contains 1 mg o-phthaldialdehyde (P0657) per mL solution with 2-mercaptoethanol as the sulfhydryl moiety.

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Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1B - Skin Sens. 1

WGK

WGK 3


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Silke B Lohan et al.
Antioxidants (Basel, Switzerland), 9(6) (2020-06-25)
The daily consumption of tobacco products leads to a boost in free radical production in tissues, promoting the risk for malignancies, metabolic alterations and chronic-inflammatory diseases. This study aimed to broaden the knowledge of the status of the antioxidative (AO)
Katsuhiro Suzuki et al.
Biomedical chromatography : BMC, 27(4), 535-538 (2012-09-29)
Monomethylarginine, asymmetric dimethylarginine and symmetric dimethylarginine were separated on a Wakopak Combi ODS with an acetonitrile-100 mm potassium phosphate buffer (pH 7.0; 1:1, v/v). Dimethylarginines were derived from o-phthalaldehyde for the fluorescence detector and from 6-ferrocenyl-1-hexanethiol for the electrochemical detector. The
Noriko Nishino et al.
Environmental science & technology, 46(15), 8198-8204 (2012-07-20)
Naphthalene, typically the most abundant polycyclic aromatic hydrocarbon in the atmosphere, reacts with OH radicals by addition to form OH-naphthalene adducts. These OH-naphthalene adducts react with O(2) and NO(2), with the two reactions being of equal importance in air at
Quansong Li et al.
Physical chemistry chemical physics : PCCP, 14(18), 6561-6568 (2012-03-30)
The potential energy surface for the intramolecular excited state hydrogen transfer (IESHT) in ortho-phthalaldehyde (OPA), which generates an enol ketene, has been studied with ab initio calculations (MS-CASPT2//CASSCF). The goal of our study is to establish the mechanistic factors that
Vishnu Menon et al.
Journal of fluorescence, 23(2), 311-321 (2012-12-06)
This is the first report of inactivation of xyloglucanase from Thermomonospora sp by pepstatin A, a specific inhibitor towards aspartic proteases. The steady state kinetics revealed a reversible, competitive, two-step inhibition mechanism with IC 50 and K i values of

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