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D9564

Sigma-Aldrich

DAPI, dilactate

DAPI, dilactate
1 of 1 reviewers received a sample product or took part in a promotion

≥98% purity (HPLC), powder

Synonym(s):

4′,6-Diamidino-2-phenyindole, dilactate

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10 MG
£146.00

£146.00

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10 MG
£146.00

About This Item

Empirical Formula (Hill Notation):
C16H15N5 · 2C3H6O3
Molecular Weight:
457.48
MDL number:
MFCD00467366
UNSPSC Code:
12171500
PubChem Substance ID:
329798905
NACRES:
NA.47

£146.00

Please contact Customer Service for Availability
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Product Name

DAPI, dilactate, ≥98% (HPLC)

Quality Level

300

Assay

≥98% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow

solubility

H2O: 20 mg/mL

ε (extinction coefficient)

28.00-32.50 at 345-351 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)

InChI key

LCAUPANIXBMNIA-UHFFFAOYSA-N

General description

DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.

Application

DAPI, dilactate has been used for the nuclear staining in cells.[1][2] DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.

Biochem/physiol Actions

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000406681
0000406681
0000384458
0000384458
0000352352

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Shahrzad Bahrampour et al.
PLoS biology, 17(2), e3000163-e3000163 (2019-02-27)
During central nervous system (CNS) development, genetic programs establish neural stem cells and drive both stem and daughter cell proliferation. However, the prominent anterior expansion of the CNS implies anterior-posterior (A-P) modulation of these programs. In Drosophila, a set of
Expression and high glucose-mediated regulation of K+ channel interacting protein 3 (KChIP3) and KV4 channels in retinal Muller glial cells.
Chavira-Suarez E, et al.
Biochemical and Biophysical Research Communications, 404, 678-683 (2011)
Vicky Goler-Baron et al.
PloS one, 6(1), e16007-e16007 (2011-02-02)
Multidrug resistance (MDR) is a major impediment to curative cancer chemotherapy. The ATP-Binding Cassette transporters ABCG2, ABCB1 and ABCC2 form a unique defense network against multiple structurally and functionally distinct chemotherapeutics, thereby resulting in MDR. Thus, deciphering novel mechanisms of
Nicolas Buchon et al.
BMC biology, 8, 152-152 (2010-12-24)
Gut homeostasis is central to whole organism health, and its disruption is associated with a broad range of pathologies. Following damage, complex physiological events are required in the gut to maintain proper homeostasis. Previously, we demonstrated that ingestion of a
Transplantation reveals regional differences in oligodendrocyte differentiation in the adult brain.
Vigano F, et al.
Nature Neuroscience, 16, 1370-1372 (2013)
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Questions

1–4 of 4 Questions  

  1. Which filter sets can be used to visualize the fluorophore produced by Product D9564, DAPI, dilactate (DAPI-DNA)?

    1 answer

    1. One such filter set is the Zeiss Filter Set 01 (ex=359 nm, em=461 nm).

      Helpful?

  2. Can Product D9564, DAPI dilactate (DAPI-DNA), be used as a vital stain?

    1 answer

    1. As we note in the Product Information Sheet for the Dihydrochloride salt of DAPI, Product No. D9542, DAPI can be used as a vital dye to stain mature pollen grains, but we have tested it as a vital stain in other cell types.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Isn't the hydrochloride salt form of DAPI a more sensitive detector of DNA than Product D9564, DAPI, dilactate (DAPI-DNA)?

    1 answer

    1. The dilactate salt has as much sensitivity in detection as the dihydrochloride salt, which we also offer as Product No. D9542.

      Helpful?

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

  1. St. Louis, MO
    • Review 1
    • Votes 0
    4 out of 5 stars.

    Good Quality

    Arrived quickly. Labeling is dimmer than expected.

    Helpful?

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