861405
Hoechst 33258
Synonym(s):
2′-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride hydrate, bisBenzimide
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About This Item
Empirical Formula (Hill Notation):
C25H24N6O · 3HCl · xH2O
CAS Number:
Molecular Weight:
533.88 (anhydrous basis)
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
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Assay
≥98% (TLC)
form
powder
mp
>300 °C (lit.)
λmax
343 nm
SMILES string
Cl[H].Cl[H].Cl[H].[H]O[H].CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5[nH]c(nc5c4)-c6ccc(O)cc6
Other Notes
This product has been replaced by B2883-Sigma-Aldrich | bisBenzimide H 33258 ≥98% (HPLC and TLC)
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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The increase in fluorescence, upon interaction with several fluorescent dyes was found to depend on the base composition of DNA. 4',6-Diamidino-2-phenylindole-2 HCl and Hoechst 33258 which bind to AT base pairs show a logarithmic relation. This relation is linear when
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