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48912-U

Sigma-Aldrich

ProClin™ 300

pkg of 50 mL, ProClin™ preservative for use in IVD manufacturing, CMIT/MIT 3 %

Synonym(s):

3% solution of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-Methyl-4-isothiazolin-3-one

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50 ML
£59.80

£59.80

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50 ML
£59.80

About This Item

UNSPSC Code:
12352201
NACRES:
NA.21

£59.80

In StockDetails

Request a Bulk Order

Quality Level

300

form

liquid

shelf life

3 yr

contains

Alkyl Carboxylate

composition

CMIT/MIT, 3%

packaging

pkg of 50 mL

parameter

<45 °C operating temperature

color

colorless to light yellow

matrix

Modified glycol

operating pH range

2.5-8.5

pH

4.1 (100 g/L)

bp

189 °C

mp

-40 °C

solubility

water: soluble

density

1.03 g/cm3

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General description

ProClin™ preservatives are water-soluble preparations of biocides that are generally used to increase the shelf life of reagents. ProClin™ 300 comprises of 3% of 5-chloro-2-methyl-4-isothiazolin-3-one (CMIT) and 2-methyl-4-isothiazloin-3-one (MIT) in a salt-free proprietary glycol containing an alkly carboxylate stabilizer. It has a recommended working pH range as 2.5 - 8.5.

Application

For use in IVD Manufacturing
ProClin™ 300 is suitable for preparation of different buffers like ELISA buffer, sample and substrate dilution buffers for Single-Molecule Arrays (Simoa assay) and Immunoenzymatic assay.[1][2] Additionally, Proclin™ may be used in preparing storage buffer to store beads coupled with coronavirus nucleocapsid protein for the serological multiplex assay.[3]
ProClin™ biocides preservatives are immediately microstatic upon contact with microbial organisms as a result of their ability to quickly penetrate cell membranes and inhibit specific enzymes crucial to cellular respiration. Multiple specific sites of ProClin™ toxicity prevent microorganisms from developing high levels of resistance, making ProClin™ highly efficient even over extended storage periods of your IVD components.

Biochem/physiol Actions

ProClin™ products contain active substances like CMIT and MIT which inhibit the Krebs cycle at four different check points, the enzymes pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, succinate dehydrogenase, and NADH dehydrogenase. The inhibition of Krebs cycle leads to effective broad-spectrum biocidal activity against bacteria, fungi, and yeast.

Legal Information

ProClin is a trademark of LANXESS Corp.

Disclaimer

The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada′s Pest Management Regulatory Agency, Turkey′s Biocidal Products Regulation, Korea′s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

244.4 °F - closed cup

Flash Point(C)

118 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCW5719
MKCW1837
MKCV4372
MKCV6710
MKCT2079

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Find documentation for the products that you have recently purchased in the Document Library.

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Annika Nelde et al.
Nature immunology, 22(1), 74-85 (2020-10-02)
T cell immunity is central for the control of viral infections. To characterize T cell immunity, but also for the development of vaccines, identification of exact viral T cell epitopes is fundamental. Here we identify and characterize multiple dominant and
Molecular-biological problems of drug design and mechanism of drug action
GI Khvatova and AV Semeikin
Journal of Pharmacy and Chemistry, 44(12), 651-653 (2011)
Single Molecule Arrays for ultra-sensitive detection of rat cytokines in serum
Cohen L, et al.
Journal of Immunological Methods, 452, 20-25 (2018)
Limor Cohen et al.
Journal of immunological methods, 452, 20-25 (2017-10-19)
Rats are used as animal models for many human diseases. Cytokines can serve as biomarkers indicative of these diseases or disease states. Techniques for measuring cytokine expression levels often do not provide the sensitivity needed to measure these biomarkers in
SARS-CoV-2 T-cell epitopes define heterologous and COVID-19-induced T-cell recognition
Nelde A, et al.
Research (Washington, D.C.) (2020)
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Questions

1–10 of 14 Questions  

  1. Is there more information about the modified alkyl carboxylate matrix used for ProClin 300?

    1 answer

    1. The product is non-hazardous, the exact chemical composition of the modified alkyl carboxylate used in ProClin 300 is a proprietary information and hence not disclosed.

      Helpful?

  2. To use as a preservative for a buffer in an RUO kit, what are the reasons for using 48912-U over 46878-U?

    1 answer

    1. Both ProClin 300 and Proclin 950 are only sold for use in In Vitro Diagnostic Use. Their usage is not allowed for Research Use Only (RUO).

      Helpful?

  3. Is the recommended level for ProClin usage (0.025 to 0.05%) based on the active ingredient or the product as it is supplied in the bottle?

    1 answer

    1. The percent of active ingredients found packaged in the bottle is 3% as it is supplied in the bottle. The recommended usage range for ProClin 300 is 0.025% to 0.050%, based on the product. Thus, an active range for use is represented between 7.5 to 15 ppm.

      Helpful?

  4. What is the process of neutralizing ProClin, and can ProClin be safely disposed of as non-hazardous after neutralization?

    1 answer

    1. ProClin 300 contains two active ingredients, CMIT and MIT. CMIT can degrade into non-hazardous by-products. MIT is less reactive and is degraded by various chemicals. The provided procedure degrades both the CMIT and MIT active ingredients, ensuring that once the solution is neutralized, CMIT and MIT are no longer present. The breakdown by-products are not hazardous.

      Helpful?

  5. Does anything in 48912-U interfere with Horseradish Peroxidase?

    1 answer

    1. ProClin 300 is fully compatible with both horseradish peroxidase and alkaline phosphatase enzymes.

      Helpful?

  6. Proclin P30 preservative - what is the recommended shelf life from DOM?

    1 answer

    1. The expiration date will be located on the lot-specific Certificate of Analysis.

      Please access a Certificate in the DOCUMENTATION section of the product detail page:
      https://www.sigmaaldrich.com/product/sial/48912-u#product-documentation

      Helpful?

  7. Can I use proclin 300 for Blood and Plasma preservation? And can i use proclin 300 with K3 EDTA?

    1 answer

    1. This product is used as a preservative in numerous products and preparations. Although it has been utilized in antibody preparations of ascites fluid, it is typically not used as a biocidal agent in whole blood, serum, or plasma. Suitability would have to be determined by the researcher. See the link below to review additional ProClin information:
      https://www.sigmaaldrich.com/products/clinical-diagnostics/proclin-preservatives

      Helpful?

  8. What is the working solution of ProClin? How to dilute ProClin?

    1 answer

    1. While optimization may be required, the typical working range for ProClin 300 is 0.03 - 0.05%. See the link below for additional information on ProCliin product usage. Scroll down for the table referencing usage concentrations:
      https://www.sigmaaldrich.com/products/clinical-diagnostics/proclin-preservatives.

      Helpful?

  9. What is the concentration of active ingredients in ProClin 300?

    1 answer

    1. ProClin 300 contains a total of 3% active ingredient.

      Helpful?

  10. When would I choose to use ProClin 300?

    1 answer

    1. ProClin 300 is best used with production runs of standards, controls, and large volume buffers and solutions.  The product is quite viscous and difficult to work with when trying to measure small volumes.

      Helpful?

1–10 of 14 Questions  

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