14780
Bis(1,3-dibutylbarbituric acid)pentamethine oxonol
for fluorescence, ≥90.0% (HPLC)
Synonym(s):
DiBAC4(5)
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About This Item
Empirical Formula (Hill Notation):
C29H42N4O6
CAS Number:
Molecular Weight:
542.67
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
grade
for fluorescence
Assay
≥90.0% (HPLC)
drug control
regulated under CDSA - not available from Sigma-Aldrich Canada
solubility
ethanol: soluble
fluorescence
λex 590 nm; λem 616 nm in ethanol
suitability
suitable for fluorescence
SMILES string
CCCCN1C(=O)C(\C=C\C=C\C=C2/C(=O)N(CCCC)C(=O)N(CCCC)C2=O)C(=O)N(CCCC)C1=O
Application
DiBAC4(5) is an anionic, slow-response potential-sensitive probe with pH insensitive fluorescence properties (excitation maxima of 590 nm and emission maxima of 616 nm) over a pH range of 4.5 to 7.5. It can enter depolarized cells and then bind to intracellular proteins or membranes. Once bound, the fluorescence properties of DiBAC4(5) are enhanced and shifted toward red. Increased cellular depolarization results in an increased influx of dye and an increase in fluorescence. The negative charge of DiBAC4(5) prevents it′s entry into mitochondria .
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
Oxonol dye which inhibits band 3-mediated anion exchange in erythrocytes
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P A Knauf et al.
The American journal of physiology, 269(4 Pt 1), C1073-C1077 (1995-10-01)
When cells are acutely exposed to the oxonol dye, bis(1,3-dibutylbarbituric acid)pentamethine oxonol (diBA), at 0 degrees C, the concentration that gives half inhibition of Cl- exchange (IC50) is 0.146 +/- 0.013 microM (n = 12) initially, but the inhibition increases
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