14780

Bis(1,3-dibutylbarbituric acid)pentamethine oxonol

for fluorescence, ≥90.0% (HPLC)

• Synonym(s): DiBAC₄(5)
• Empirical Formula (Hill Notation): C₂₉H₄₂N₄O₆
• CAS Number: 63560-89-4
• Molecular Weight: 542.67
• MDL number: MFCD07437856
• UNSPSC Code: 12352200
• PubChem Substance ID: 24848828

Properties

• grade: for fluorescence
• Assay: ≥90.0% (HPLC)
• drug control: regulated under CDSA - not available from Sigma-Aldrich Canada
• solubility: ethanol: soluble
• fluorescence: λ_ex 590 nm; λ_em 616 nm in ethanol
• SMILES string: CCCCN1C(=O)C(\C=C\C=C\C=C2/C(=O)N(CCCC)C(=O)N(CCCC)C2=O)C(=O)N(CCCC)C1=O

Description

Application

DiBAC4(5) is an anionic, slow-response potential-sensitive probe with pH insensitive fluorescence properties (excitation maxima of 590 nm and emission maxima of 616 nm) over a pH range of 4.5 to 7.5. It can enter depolarized cells and then bind to intracellular proteins or membranes. Once bound, the fluorescence properties of DiBAC4(5) are enhanced and shifted toward red. Increased cellular depolarization results in an increased influx of dye and an increase in fluorescence. The negative charge of DiBAC4(5) prevents it′s entry into mitochondria .

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Oxonol dye which inhibits band 3-mediated anion exchange in erythrocytes[1]