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SCT120

Sigma-Aldrich

BioTracker 488 Green Nuclear Dye

BioTracker 488 Green Nuclear Dye

Live cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.

Synonym(s):

Live cell imaging probe, Live cell nuclear imaging probe

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1 KIT
£363.00

£363.00

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1 KIT
£363.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.47

£363.00

In StockDetails

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

General description

The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell′s genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.

The BioTracker 488 Green Nuclear Dye is a cell-membrane permeable green fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, and it has low toxicity for live cell imaging. The dye is supplied with a vial of verapamil, an efflux pump inhibitor that may improve probe retention and live cell staining in certain cell types.

Note: BioTracker Nuclear Dyes also shows blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.

Spectral Properties
Absorbance: 500nm
Emission: 515nm

Application

Live cell fluorescent imaging
Live cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Components

1) 1 vial of 50µL BioTracker 488 Green Nuclear Dye (1000X in DMSO) (CS224594)

2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)

Quality

Spectral Properties
Absorbance: 500nm
Emission: 515nm

Physical form

Liquid

Storage and Stability

Store BioTracker 488 Green Nuclear Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H302,H411

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

1–7 of 7 Questions  

  1. Does the fluorescence intensity increase by chromosome condensation similarly to DAPI or Hoechst?

    1 answer

    1. This product has not been tested for an increase in fluorescence intensity with chromosome condensation.

      Helpful?

  2. Is there any toxicity data available for extended (>48h) live cell staining?

    1 answer

    1. The dye with verapamil showed no obvious toxicity in MCF-7 cells over the course of 72 hours. It may be different for different cell lines and different experimental conditions.

      Helpful?

  3. Is it possible to use SCT120 BioTracker 488 Green Nuclear Dye and PKH26 Red Fluorescent Cell Linker for in vivo applications, and what is their stability duration?

    1 answer

    1. The PKH series of dyes are designed for membrane labeling and can be used to track cells through several divisions. The intensity of the dye decreases by half with each cell division, so the duration of visibility will depend on the cell division rate of the specific cell type.

      Regarding SCT120 nuclear dye, there is limited information about the viability of cells after staining or the photostability of the dye once the cells have been stained.

      Currently, there is no available information on the compatibility or effects of using SCT120 in conjunction with PKH26GL for dual staining of cells.

      Helpful?

  4. How can I optimize staining using SCT120?

    1 answer

    1. The recommended protocol for using SCT120 is as follows:

      1. Dilute the BioTracker™ Nuclear Dye from its 1000X stock solution to a working concentration of 1X in cell culture medium. To achieve this, one could add 1 µL of dye to 1 mL of culture medium. It is important to note that the optimal concentration of the probe may vary depending on the cell type. Optionally, verapamil can be included in the staining solution to enhance probe retention in live cells. The ideal concentration of verapamil will likely be different across cell types, and it is suggested to test concentrations ranging from 10-100 µM.

      2. Discard the culture medium from the cells and replace it with the diluted BioTracker™ Nuclear Dye solution. Incubate the cells at 37°C for a minimum of 10 minutes.

      To optimize staining, consider the following adjustments:

      • Probe Dilution: Although the standard dilution is 1:1000, experimenting with other dilutions such as 1:500 or 1:750 could be beneficial. Observe the results and, if necessary, test additional dilutions to find the most effective concentration.

      • Verapamil Concentration: The suggested range for verapamil is between 10 to 100 µM. Trial with two or three different dilutions within this spectrum may help to refine the staining process.

      • Incubation Time: Initially, a 10-minute incubation is recommended. However, extending this period to 20, 40, or even 60 minutes at 37°C could improve staining results. If positive changes are noted, further experimentation with the duration may be warranted to optimize the staining signal.

      It is vital to only change one variable at a time during these optimizations. Altering multiple factors simultaneously, such as the dye dilution, verapamil concentration, and incubation time, would make it difficult to identify which specific change led to any observed improvements. Additionally, checking cell viability before staining is advisable. Using Trypan Blue to determine cell viability might provide insights into the overall health and suitability of the cells for staining.

      Helpful?

  5. Will SCT120, BioTracker 488 Green Nuclear Dye SCT work with the FITC channel?

    Will SCT120, BioTracker 488 Green Nuclear Dye SCT work with the FITC channel?

    1 answer

    1. Yes, for SCT120 we do recommend analyzing the cells using the FITC channel.

      Please refer to the product's data sheet, found in the DOCUMENTATION section of the Product Detail Page: https://www.sigmaaldrich.com/product/mm/sct120#product-documentation

      Helpful?

  6. Could you please provide the chemical structure and formula for BIOTRACKER 488 GREEN NUCLEAR DYE, if available?

    1 answer

    1. This information is considered proprietary.

      Helpful?

  7. Hi I was wondering if this stain would work in a 100% alcoholic liquid, or whether it requires protons to fluoresce?

    1 answer

    1. The dye has not been validated for DNA binding under 100% alcoholic liquid. It does not require protons to fluoresce.

      Helpful?

Reviews

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  1. Lewiston, ME
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Great!

    Works perfectly for my application with accurate results.

    Helpful?

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