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SCR012

Sigma-Aldrich

PluriStem 129/S6 Murine ES cells

The PluriStem 129S6 Murine ES cell line is derived from the widely used 129/S6/SvEv strain of mice (at 3.5 days p.c.) & is intended for injection into black or albino host blastocysts.

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About This Item

UNSPSC Code:
41106509
eCl@ss:
32011203
NACRES:
NA.81

biological source

mouse

Quality Level

manufacturer/tradename

Chemicon®
PluriSTEM®

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)

shipped in

liquid nitrogen

General description

The PluriStem 129S6 Murine ES cell line is derived from the widely used 129/S6/SvEv strain of mice (at 3.5 days p.c.) and is intended for injection into black or albino host blastocysts. This cell line is characterized by the production of highly chimeric, germline-competent mice. Cells are supplied at passage 10.

Complete disease testing and karyotype data is available under Information Library tool bar, Tech Notes at SCR012 product specific page at http://www.chemicon.com/Product/ProductDataSheet.asp?ProductItem=SCR012.

STRAIN: 129/S6/SvEv

GENDER: Male

KARYOTYPE: 40XY

Cell Line Description

Embryonic Stem Cells

Application

Research Category
Stem Cell Research

Packaging

2.5 x 106 cells per vial

Physical form

Cells are supplied frozen in basal medium supplemented with 10% FCS and 10% DMSO

Storage and Stability

Place vials in liquid Nitrogen immediately upon receipt until it is convenient to proceed to subculture.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

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Ting Li et al.
Oncotarget, 6(31), 31805-31819 (2015-09-18)
Activation of IκB kinase β (IKK-β) and nuclear factor (NF)-κB signaling contributes to cancer pathogenesis and inflammatory disease; therefore, the IKK-β-NF-κB signaling pathway is a potential therapeutic target. Current drug design strategies focus on blocking NF-κB signaling by binding to
Yang Gao et al.
Journal of neurochemistry, 134(2), 340-353 (2015-04-16)
Glutamate carboxypeptidase II (GCPII) is a transmembrane zinc metallopeptidase found mainly in the nervous system, prostate and small intestine. In the nervous system, glia-bound GCPII mediates the hydrolysis of the neurotransmitter N-acetylaspartylglutamate (NAAG) into glutamate and N-acetylaspartate. Inhibition of GCPII
Jianqiao Zhong et al.
BioMed research international, 2016, 6894743-6894743 (2016-09-17)
Bcl-2 and Nrf2 are critical factors in protecting cells against UVB-induced apoptosis. Hair-follicle-bulge stem cells could resist ionization through Bcl-2 upregulation. Skin-derived precursors (SKPs) dwelling on the bulge may be against UVB irradiation. Initially, SKPs were isolated and identified. Then
Ruilin Sun et al.
Nucleic acids research, 40(21), e166-e166 (2012-08-11)
Methods for generating loss-of-function mutations, such as conventional or conditional gene knockout, are widely used in deciphering gene function in vivo. By contrast, inducible and reversible regulation of endogenous gene expression has not been well established. Using a mouse model
Tao Deng et al.
Development, growth & differentiation, 49(7), 603-610 (2007-07-20)
The completely embryonic stem (ES) cell-derived mice (ES mice) produced by tetraploid embryo complementation provide us with a rapid and powerful approach for functional genome analysis. However, inbred ES cell lines often fail to generate ES mice. The genome of

Protocols

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

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